| Transcriptomic and metabolic analysis of an astaxanthin-hyperproducing Haematococcus pluvialis mutant obtained by low-temperature plasma (LTP) mutagenesis under high light irradiation | |
Chen, Z; Chen, J; Liu, JH; Li, LM; Qin, S ; Huang, Q
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| 发表期刊 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| ISSN | 2211-9264 |
| 2020-01 | |
| 卷号 | 45页码:101746 |
| 关键词 | Haematococcus pluvialis Astaxanthin High light irradiation Transcriptome Mutant Photosynthesis |
| 研究领域 | Biotechnology & Applied Microbiology |
| DOI | 10.1016/j.algal.2019.101746 |
| 产权排序 | [Chen, Zhu; Liu, Jinghua; Li, Lamei; Huang, Qing] Chinese Acad Sci, Inst Tech Biol & Agr Engn, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China; [Chen, Zhu; Liu, Jinghua; Li, Lamei; Huang, Qing] Univ Sci & Technol China, Hefei 230026, Peoples R China; [Chen, Jun; Qin, Song] Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Jinghua] Anhui Sci & Technol Univ, Coll Life Sci, Fengyang 233100, Peoples R China |
| 通讯作者 | Huang, Qing(huangq@ipp.ac.cn) |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Haematococcus pluvialis can accumulate sufficient levels of astaxanthin under various stress conditions. High light irradiation is essential for astaxanthin accumulation in Haematococcus pluvialis. However, a lack of genomic information limits the understanding of its physiological metabolism for high astaxanthin production. In this work, we investigated the wild-type (WT) strain and mutant strain (named as M3 which was obtained in our previous work) under high light irradiation and compared the difference in astaxanthin biosynthesis. We collected and analyzed the data of de novo transcriptome information of Haematococcus pluvialis at 24 h under high light stress. Our transcriptomic results indicated that M3 strain had higher utilization efficiency of CO2 to provide the precursors of carotenoid and fatty acid biosynthesis by increasing the expression levels of phosphoenolpyruvate carboxylase (PEPC), malate dehydrogenase (MDH), malate dehydrogenase (ME), ribulose bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) while decreasing the expression levels of fructose-1, 6-bisphosphatase (FBP). The analysis of pigments, chlorophyll fluorescence and the quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that M3 strain maintained higher photosynthetic activity by regulating chlororespiration pathway and elevating non-photosynthetic pigment (lutein, beta-carotene, and astaxanthin) content to alleviate photooxidative damage. Moreover, the M3 strain showed higher fatty acid content than the WT strain. Overall, combinative analysis of de novo transcriptomic and physiological data provided information necessary for not only a better understanding of the difference in astaxanthin biosynthesis between WT and M3 strains but also the feasibility of genetic engineering of Haematococcus pluvialis in the future. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [11775272, 11635013, 11475217] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CAROTENOID BIOSYNTHESIS GENES ; CHLOROPHYLL FLUORESCENCE ; SECONDARY CAROTENOIDS ; HARVESTING COMPLEX ; BETA-CAROTENE ; STRESS ; ACCUMULATION ; EXPRESSION ; MICROALGAE ; RUBISCO |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000507580300044 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24781 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Tech Biol & Agr Engn, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China; 2.Univ Sci & Technol China, Hefei 230026, Peoples R China; 3.Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 4.Anhui Sci & Technol Univ, Coll Life Sci, Fengyang 233100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Z,Chen, J,Liu, JH,et al. Transcriptomic and metabolic analysis of an astaxanthin-hyperproducing Haematococcus pluvialis mutant obtained by low-temperature plasma (LTP) mutagenesis under high light irradiation[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2020,45:101746. |
| APA | Chen, Z,Chen, J,Liu, JH,Li, LM,Qin, S,&Huang, Q.(2020).Transcriptomic and metabolic analysis of an astaxanthin-hyperproducing Haematococcus pluvialis mutant obtained by low-temperature plasma (LTP) mutagenesis under high light irradiation.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,45,101746. |
| MLA | Chen, Z,et al."Transcriptomic and metabolic analysis of an astaxanthin-hyperproducing Haematococcus pluvialis mutant obtained by low-temperature plasma (LTP) mutagenesis under high light irradiation".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 45(2020):101746. |
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