A full-length cDNA library was constructed from the tuber of Jerusalem artichoke by recombinating the full-length cDNAs with the Gateway donor vector of pDONR222 for gene discovery and functional characterization. The cDNA library quality analysis showed that the capacity of original library was 5.76×106 CFU. The inserted fragments were mainly in the range of 1~3 000 bp and the recombination rate was 100% (24/24). These results indicated that the cDNA library had a high quality. EST sequencing was conducted and 2 639 high-quality ESTs were obtained, and 1 895 unigenes were obtained after assembly. NCBI database (NR) homologous alignment analysis showed that a total of 1 533 unigenes (80.9%) had significant homology with known genes. GO classification showed that the proportion of binding and catalytic activity were highest among the molecular function taxon. The cDNA library can be well used for functional genomics, novel gene screening, high-through EST sequencing and the preparation of cDNA microarray of Jerusalem artichoke.
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