Imaging of anti-inflammatory effects of HNO via a near-infrared fluorescent probe in cells and in rat gouty arthritis model
Huang, Yan; Zhang, Xia; He, Na; Wang, Yue; Kang, Qi; Shen, Dazhong; Yu, Fabiao; Chen, Lingxin
Source PublicationJOURNAL OF MATERIALS CHEMISTRY B
ISSN2050-750X
2019-01-14
Volume7Issue:2Pages:305-313
KeywordHYDROGEN-SULFIDE H2S NITROXYL HNO NITRIC-OXIDE AQUEOUS-SOLUTION LIVING CELLS REDUCTANT-RESISTANT ANGELIS SALT MITOCHONDRIA INFLAMMATION VISUALIZATION
MOST Discipline CatalogueMaterials Science
DOI10.1039/c8tb02494d
Contribution Rank[Huang, Yan; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Minist Educ, Key Lab Mol & Nanoprobes, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China; [Huang, Yan; Zhang, Xia; He, Na; Wang, Yue; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Zhang, Xia; Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Fabiao] Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
Department海岸带环境过程实验室
AbstractNitroxyl (HNO) plays a crucial role in anti-inflammatory effects via the inhibition of inflammatory pathways, but the details of the endogenous generation of HNO still remain challenging owing to the complex biosynthetic pathways, in which the interaction between H2S and NO simultaneously generates HNO and polysulfides (H2Sn) in mitochondria. Moreover, nearly all the available fluorescent probes for HNO are utilized for imaging HNO in cells and tissues, instead of the in situ real-time detection of the simultaneous formation of HNO and H2Sn in mitochondria and animals. Here, we have developed a mitochondria-targeting near-infrared fluorescent probe, namely, Mito-JN, to detect the generation of HNO in cells and a rat model. The probe consists of three moieties: Aza-BODIPY as a fluorescent signal transducer, a triphenylphosphonium cation as a mitochondria-targeting agent, and a diphenylphosphinobenzoyl group as an HNO-responsive unit. The response mechanism is based on an aza-ylide intramolecular ester aminolysis reaction with fluorescence emissions on. Mito-JN displays high selectivity and sensitivity for HNO over various other biologically relevant species. Mito-JN was successfully used for the detection of the endogenous generation of HNO, which is derived from the crosstalk between H2S and NO in living cells. The additional generation of H2Sn was also confirmed using our previous probe Cy-Mito. The anti-inflammatory effect of HNO was examined in a cell model of LPS-induced inflammation and a rat model of gouty arthritis. The results imply that our probe is a good candidate for the assessment of the protective effects of HNO in inflammatory processes.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordHYDROGEN-SULFIDE H2S ; NITROXYL HNO ; NITRIC-OXIDE ; AQUEOUS-SOLUTION ; LIVING CELLS ; REDUCTANT-RESISTANT ; ANGELIS SALT ; MITOCHONDRIA ; INFLAMMATION ; VISUALIZATION
WOS Research AreaMaterials Science, Biomaterials
WOS IDWOS:000454922000010
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24621
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
Affiliation1.Shandong Normal Univ, Minist Educ, Key Lab Mol & Nanoprobes, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
4.Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China;
5.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
Recommended Citation
GB/T 7714
Huang, Yan,Zhang, Xia,He, Na,et al. Imaging of anti-inflammatory effects of HNO via a near-infrared fluorescent probe in cells and in rat gouty arthritis model[J]. JOURNAL OF MATERIALS CHEMISTRY B,2019,7(2):305-313.
APA Huang, Yan.,Zhang, Xia.,He, Na.,Wang, Yue.,Kang, Qi.,...&Chen, Lingxin.(2019).Imaging of anti-inflammatory effects of HNO via a near-infrared fluorescent probe in cells and in rat gouty arthritis model.JOURNAL OF MATERIALS CHEMISTRY B,7(2),305-313.
MLA Huang, Yan,et al."Imaging of anti-inflammatory effects of HNO via a near-infrared fluorescent probe in cells and in rat gouty arthritis model".JOURNAL OF MATERIALS CHEMISTRY B 7.2(2019):305-313.
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