| Imaging of anti-inflammatory effects of HNO via a near-infrared fluorescent probe in cells and in rat gouty arthritis model | |
Huang, Yan; Zhang, Xia; He, Na; Wang, Yue; Kang, Qi; Shen, Dazhong; Yu, Fabiao; Chen, Lingxin
| |
| 发表期刊 | JOURNAL OF MATERIALS CHEMISTRY B
 |
| ISSN | 2050-750X |
| 2019-01-14 | |
| 卷号 | 7期号:2页码:305-313 |
| 关键词 | HYDROGEN-SULFIDE H2S NITROXYL HNO NITRIC-OXIDE AQUEOUS-SOLUTION LIVING CELLS REDUCTANT-RESISTANT ANGELIS SALT MITOCHONDRIA INFLAMMATION VISUALIZATION |
| 研究领域 | Materials Science |
| DOI | 10.1039/c8tb02494d |
| 产权排序 | [Huang, Yan; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Minist Educ, Key Lab Mol & Nanoprobes, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China; [Huang, Yan; Zhang, Xia; He, Na; Wang, Yue; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Zhang, Xia; Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Fabiao] Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China |
| 作者部门 | 海岸带环境过程实验室 |
| 英文摘要 | Nitroxyl (HNO) plays a crucial role in anti-inflammatory effects via the inhibition of inflammatory pathways, but the details of the endogenous generation of HNO still remain challenging owing to the complex biosynthetic pathways, in which the interaction between H2S and NO simultaneously generates HNO and polysulfides (H2Sn) in mitochondria. Moreover, nearly all the available fluorescent probes for HNO are utilized for imaging HNO in cells and tissues, instead of the in situ real-time detection of the simultaneous formation of HNO and H2Sn in mitochondria and animals. Here, we have developed a mitochondria-targeting near-infrared fluorescent probe, namely, Mito-JN, to detect the generation of HNO in cells and a rat model. The probe consists of three moieties: Aza-BODIPY as a fluorescent signal transducer, a triphenylphosphonium cation as a mitochondria-targeting agent, and a diphenylphosphinobenzoyl group as an HNO-responsive unit. The response mechanism is based on an aza-ylide intramolecular ester aminolysis reaction with fluorescence emissions on. Mito-JN displays high selectivity and sensitivity for HNO over various other biologically relevant species. Mito-JN was successfully used for the detection of the endogenous generation of HNO, which is derived from the crosstalk between H2S and NO in living cells. The additional generation of H2Sn was also confirmed using our previous probe Cy-Mito. The anti-inflammatory effect of HNO was examined in a cell model of LPS-induced inflammation and a rat model of gouty arthritis. The results imply that our probe is a good candidate for the assessment of the protective effects of HNO in inflammatory processes. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [21575159, 21775162, 21405172, 21575080] ; Youth Innovation Promotion Association, CAS [2015170] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | HYDROGEN-SULFIDE H2S ; NITROXYL HNO ; NITRIC-OXIDE ; AQUEOUS-SOLUTION ; LIVING CELLS ; REDUCTANT-RESISTANT ; ANGELIS SALT ; MITOCHONDRIA ; INFLAMMATION ; VISUALIZATION |
| 研究领域[WOS] | Materials Science, Biomaterials |
| WOS记录号 | WOS:000454922000010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24621 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Shandong Normal Univ, Minist Educ, Key Lab Mol & Nanoprobes, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China; 5.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Yan,Zhang, Xia,He, Na,et al. Imaging of anti-inflammatory effects of HNO via a near-infrared fluorescent probe in cells and in rat gouty arthritis model[J]. JOURNAL OF MATERIALS CHEMISTRY B,2019,7(2):305-313. |
| APA | Huang, Yan.,Zhang, Xia.,He, Na.,Wang, Yue.,Kang, Qi.,...&Chen, Lingxin.(2019).Imaging of anti-inflammatory effects of HNO via a near-infrared fluorescent probe in cells and in rat gouty arthritis model.JOURNAL OF MATERIALS CHEMISTRY B,7(2),305-313. |
| MLA | Huang, Yan,et al."Imaging of anti-inflammatory effects of HNO via a near-infrared fluorescent probe in cells and in rat gouty arthritis model".JOURNAL OF MATERIALS CHEMISTRY B 7.2(2019):305-313. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Imaging of anti-infl（2827KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论