Biodegradation of 2,6-dibromo-4-nitrophenol by Cupriavidus sp strain CNP-8: Kinetics, pathway, genetic and biochemical characterization
Min, Jun; Chen, Weiwei; Hu, Xiaoke
Source PublicationJOURNAL OF HAZARDOUS MATERIALS
ISSN0304-3894
2019-01-05
Volume361Pages:10-18
Keyword2,6-Dibromo-4-nitrophenol Biodegradation Catabolic mechanism Cupriavidus sp. FADH(2)-dependent monooxygenase
MOST Discipline CatalogueEngineering ; Environmental Sciences & Ecology
DOI10.1016/j.jhazmat.2018.08.063
Contribution Rank[Min, Jun; Chen, Weiwei; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; [Min, Jun; Hu, Xiaoke] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
Department海岸带生物学与生物资源保护实验室
AbstractCompound 2,6-dibromo-4-nitrophenol (2,6-DBNP) with high cytotoxicity and genotoxicity has been recently identified as an emerging brominated disinfection by-product during chloramination and chlorination of water, and its environmental fate is of great concern. To date, the biodegradation process of 2,6-DBNP is unknown. Herein, Cupriavidus sp. strain CNP-8 was reported to be able to utilize 2,6-DBNP as a sole source of carbon, nitrogen and energy. It degraded 2,6-DBNP in concentrations up to 0.7 mM, and the degradation of 2,6-DBNP conformed to Haldane inhibition model with mu(max) of 0.096 h(-1), K-s of 0.05 mM and K-i of 0.31 mM. Comparative transcriptome and real-time quantitative PCR analyses suggested that the hnp gene cluster was likely responsible for 2,6-DBNP catabolism. Three Hnp proteins were purified and functionally verified. HnpA, a FADH(2)-dependent monooxygenase, was found to catalyze the sequential denitration and debromination of 2,6-DBNP to 6-bromohydroxyquinol (6-BHQ) in the presence of the flavin reductase HnpB. Gene knockout and complementation revealed that hnpA is essential for strain CNP-8 to utiluze 2,6-DBNP. HnpC, a 6-BHQ 1,2-dioxygenase was proposed to catalyze the ring-cleavage of 6-BHQ during 2,6-DBNP catabolism. These results fill a gap in the understanding of the microbial degradation process and mechanism of 2,6-DBNP.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordDISINFECTION BY-PRODUCTS ; P-NITROPHENOL ; 2,4,6-TRICHLOROPHENOL DEGRADATION ; CONTAMINATED SOIL ; BIOAUGMENTATION ; CATABOLISM ; 2-CHLORO-4-NITROPHENOL ; MONOOXYGENASE ; EFFLUENTS ; CLUSTER
WOS Research AreaEngineering, Environmental ; Environmental Sciences
WOS IDWOS:000449125800002
Funding OrganizationNational Natural Science Foundation of China [31600085] ; State's Key Project of Research and Development Plan [2016YFC1402300] ; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013] ; State Key Laboratory of Microbial Metabolism, Shanghai JiaoTong University [MMLKF17-04] ; Yantai Science and Technology Project [2017ZH092]
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Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24617
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Min, Jun,Chen, Weiwei,Hu, Xiaoke. Biodegradation of 2,6-dibromo-4-nitrophenol by Cupriavidus sp strain CNP-8: Kinetics, pathway, genetic and biochemical characterization[J]. JOURNAL OF HAZARDOUS MATERIALS,2019,361:10-18.
APA Min, Jun,Chen, Weiwei,&Hu, Xiaoke.(2019).Biodegradation of 2,6-dibromo-4-nitrophenol by Cupriavidus sp strain CNP-8: Kinetics, pathway, genetic and biochemical characterization.JOURNAL OF HAZARDOUS MATERIALS,361,10-18.
MLA Min, Jun,et al."Biodegradation of 2,6-dibromo-4-nitrophenol by Cupriavidus sp strain CNP-8: Kinetics, pathway, genetic and biochemical characterization".JOURNAL OF HAZARDOUS MATERIALS 361(2019):10-18.
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