| Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum | |
Wei, Qianyu; Mu, Changkao; Wang, Chunlin; Zhao, Jianmin
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2018-10 | |
| 卷号 | 81页码:502-508 |
| 关键词 | Ruditapes philippinarum c-type lysozyme Tissue distribution Innate immunity |
| 研究领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2018.07.036 |
| 产权排序 | [Wei, Qianyu; Mu, Changkao; Wang, Chunlin] Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Wei, Qianyu] Ningbo Univ, Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315211, Zhejiang, Peoples R China |
| 作者部门 | 海岸带生物资源高效利用研究与发展中心 |
| 英文摘要 | Lysozyme is an antibacterial enzyme that is widely distributed in nature and plays an important role in the prevention of bacterial infections. In this study, a c-type lysozyme (designated as "RpCLYZ") was cloned and characterized from the manila clam, Ruditapes philippinarum. The full-length cDNA was 619 bp with an open reading frame (ORF) of 447 bp, and encoded a polypeptide of 148 amino acids with a calculated molecular mass of 17.0 kDa and an isoelectric point of 4.83. RpCLYZ was found to share high sequence similarity with c-type lysozymes from other invertebrates. The mRNA transcript of RpCLYZ was universally expressed in a wide range of tissues, especially in gills and mantle. Challenge with Vibrio anguillarum, significantly induced mRNA expression of RpCLYZ, which reached a maximum level 48 h after bacterial challenge. Recombinant RpCLYZ (rRpCLYZ) exhibited antibacterial activities against both Gram-positive and Gram-negative bacteria. Additionally, the optimal pH and temperature for rRpCLYZ activity were determined to be 4.5 and 20 degrees C, respectively. These results suggest that RpCLYZ participates in innate immune responses against bacterial invasion. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [41476126] ; Chinese Academy of Sciences [XDA11020702, XDA11020305, KZZD-EW-14] ; Youth Innovation Promotion Association CAS [2016196] ; K C Wong Magana Fund in Ningbo University |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MYTILUS-EDULIS ; CLONING ; MUSSEL |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000443665900060 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24536 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; 3.Ningbo Univ, Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315211, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Qianyu,Mu, Changkao,Wang, Chunlin,et al. Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2018,81:502-508. |
| APA | Wei, Qianyu,Mu, Changkao,Wang, Chunlin,&Zhao, Jianmin.(2018).Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,81,502-508. |
| MLA | Wei, Qianyu,et al."Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 81(2018):502-508. |
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