Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities | |
Sun Yanyu; Wang Hui; Li Junde; Wang Bin; Qi Cancan; Hu Xiaoke | |
发表期刊 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY |
ISSN | 2096-5508 |
2018-07 | |
卷号 | 36期号:4页码:1294-1303 |
关键词 | bioremediation nutrient-enhanced bacterial communities n-alkane |
研究领域 | Marine & Freshwater Biology ; Oceanography |
DOI | 10.1007/s00343-018-6310-y |
产权排序 | [Sun Yanyu; Wang Hui; Li Junde; Wang Bin; Qi Cancan; Hu Xiaoke] Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; [Sun Yanyu; Li Junde; Wang Bin; Qi Cancan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
作者部门 | 海岸带生物学与生物资源保护实验室 |
英文摘要 | Bioremediation, is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However, the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic nutrients. To understand the effects of nutrient addition on the bioremediation of crude oil and the succession of bacterial communities during process of bioremediation, microcosms containing oil-contaminated sediments were constructed and biodegradation of crude oil was assessed based on the depletion of different ingredients. We used two culture-independent methods, denaturing gradient gel electrophoresis and a 16S rRNA gene based clone library, to analyze the succession of bacterial communities. The results suggested n-alkanes were degraded after 30 days and that nutrient amendments significantly improved the efficiency of their biodegradation. Moreover, oil contamination and nutrient amendments could dramatically change bacterial community structures. Lower diversity was detected after being contaminated with oil. For instance, bacterial clones affiliated with the phylum Armatimonadetes, Firmicutes, Gemmatimonadetes, and Planctomycetes and the class Deltaproteobacteria and Epsilonproteobacteria could not be identified after 30 days of incubation with crude oil. However, "professional hydrocarbonocastic bacteria" became abundant in samples treated with oil during the bioremediation period, while these clones were almost completely absent from the control plots. Interestingly, bioinformatics analysis showed that even when dramatic differences in oil biodegradation efficiency were observed, bacterial communities in the plots with nutrient amendments were not significantly different from those in plots treated with oil alone. These findings indicated that nutrient amendments could stimulate the process of biodegradation but had less impact on bacterial communities. Overall, nutrient amendments might be able to stimulate the growth of n-alkane degrading bacteria. |
文章类型 | Article |
资助机构 | Chinese Academy of Sciences ; Chinese Academy of Sciences [KZZD-EW-14] ; Science and Technology Program of Shandong Province [2013GHY11534] ; National Natural Science Foundation of China [41376138, 41576165] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | CRUDE-OIL ; SP-NOV. ; HYDROCARBON DEGRADATION ; CONTAMINATED SOIL ; GEN. NOV. ; DEGRADING BACTERIUM ; MARINE BACTERIUM ; SP. NOV. ; BIOREMEDIATION ; MICROORGANISMS |
研究领域[WOS] | Limnology ; Oceanography |
WOS记录号 | WOS:000441231100024 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24502 |
专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Sun Yanyu,Wang Hui,Li Junde,et al. Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1294-1303. |
APA | Sun Yanyu,Wang Hui,Li Junde,Wang Bin,Qi Cancan,&Hu Xiaoke.(2018).Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1294-1303. |
MLA | Sun Yanyu,et al."Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1294-1303. |
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