| Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples | |
Su Lei ; Zhang Qianqian; Gong Jun
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| 发表期刊 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 2096-5508 |
| 2018-05 | |
| 卷号 | 36期号:3页码:818-826 |
| 关键词 | Ciliophora Peritrichia clone library internal transcribed spacer (ITS) rDNA specific PCR primers |
| 研究领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-018-6326-3 |
| 产权排序 | [Su Lei; Zhang Qianqian; Gong Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Microbial Ecol & Matter Cycles, Yantai 264003, Peoples R China; [Su Lei; Zhang Qianqian; Gong Jun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Gong Jun(jgong@yic.ac.cn) |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifications of peritrich species and assemblages in the environment are time-consuming and expertise-demanding. in this study, two peritrich-specific PCR primers were newly designed to amplify a fragment including the internal transcribed spacer (ITS) region of ribosomal rDNA from environmental samples. The primers showed high specificity in silico, and in tests with peritrich isolates and environmental DNA. Application of these primers in clone library construction and sequencing yielded exclusively sequences of peritrichs for water and sediment samples. We also found the ITS1, ITS2, ITS, D1 region of 28S rDNA, and ITS+D1 region co-varied with, and generally more variable than, the V9 region of 18S rDNA in peritrichs. The newly designed specific primers thus provide additional tools to study the molecular diversity, community composition, and phylogeography of these ecologically important protists in different systems. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [31572255, 41522604, 31301867] ; Strategic Priority Research Program of CAS [XDA11020702] ; Science and Technology Development Program of Yantai [2014ZH073] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | BIOFILM COMMUNITIES ; SEQUENCE ALIGNMENT ; WATER-QUALITY ; DIVERSITY ; RNA ; VORTICELLIDAE ; CILIOPHORA ; PROTOZOA ; SYSTEM ; CHINA |
| 研究领域[WOS] | Limnology ; Oceanography |
| WOS记录号 | WOS:000438791100020 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24468 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Microbial Ecol & Matter Cycles, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su Lei,Zhang Qianqian,Gong Jun. Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(3):818-826. |
| APA | Su Lei,Zhang Qianqian,&Gong Jun.(2018).Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(3),818-826. |
| MLA | Su Lei,et al."Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.3(2018):818-826. |
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| Development and eval（639KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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