| Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater | |
Li, Jinhua ; Fu, Junqing; Yang, Qian; Wang, Liyan; Wang, Xiaoyan; Chen, Lingxin
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| 发表期刊 | ANALYST
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| ISSN | 0003-2654 |
| 2018-08-07 | |
| 卷号 | 143期号:15页码:3570-3578 |
| 关键词 | TURN-ON POLYMER NANOPARTICLES MICROSPHERES |
| 研究领域 | Chemistry |
| DOI | 10.1039/c8an00811f |
| 产权排序 | [Li, Jinhua; Yang, Qian; Wang, Liyan; Wang, Xiaoyan; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Fu, Junqing] Prov Food & Drug Inspect Inst Shandong, Jinan 250101, Shandong, Peoples R China; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Chen, Lingxin] Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China |
| 通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) |
| 作者部门 | 海岸带环境工程技术研究与发展中心 |
| 英文摘要 | Versatile molecular imprinted fluorescence sensors have been prepared for various species, but the imprinting based fluorescence detection of target proteins upon different external stimulation is rarely reported. Herein, a novel phycocyanin-imprinted ratiometric fluorescence nanosensor was developed for the temperature regulated sensing and detection of a phycocyanin target based on fluorescence resonance energy transfer (FRET). The nanosensor was fabricated via simple facile copolymerization, with amino/carboxyl modified quantum dots (QDs) as a fluorescent support, N-isopropylacrylamide as a thermo-responsive functional monomer, N,N-methylenebisacrylamide as a cross-linker and phycocyanin as a template. Under temperature control at 20 degrees C and 45 degrees C, the fluorescence intensities of the QDs and phycocyanin were regularly decreased and enhanced, respectively, to a different extent as the concentration of phycocyanin increased, and thereby the ratio of the two fluorescence peak emission intensities of QDs and phycocyanin was used to determine the concentration of phycocyanin. Good linearity was obtained within the range of 0-1.8 M (r = 0.9900) with a low detection limit of 3.2 nM, and excellent recognition selectivity towards the phycocyanin target was achieved over other proteins. Moreover, satisfactory recoveries of 92.0-106.8% were obtained in spiked seawater samples. This study provides a facile, fast and intelligent way to conduct identification analysis of trace proteins in complex water matrices, and can push forward protein imprinting and stimuli-responsive imprinting related research. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [21477160, 41776110] ; Natural Science Foundation of Shandong Province of China [ZR2016BL25, ZR2016DB07] ; National Defence Science and Technology Innovation Project of the Chinese Academy of Sciences [CXJJ-16M254] ; Department of Science and Technology of Shandong Province of China [GG201709290055] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | TURN-ON ; POLYMER ; NANOPARTICLES ; MICROSPHERES |
| 研究领域[WOS] | Chemistry, Analytical |
| WOS记录号 | WOS:000439586400008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24426 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Prov Food & Drug Inspect Inst Shandong, Jinan 250101, Shandong, Peoples R China; 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; 4.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China; 5.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jinhua,Fu, Junqing,Yang, Qian,et al. Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater[J]. ANALYST,2018,143(15):3570-3578. |
| APA | Li, Jinhua,Fu, Junqing,Yang, Qian,Wang, Liyan,Wang, Xiaoyan,&Chen, Lingxin.(2018).Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater.ANALYST,143(15),3570-3578. |
| MLA | Li, Jinhua,et al."Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater".ANALYST 143.15(2018):3570-3578. |
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