| Necessity of electrically conductive pili for methanogenesis with magnetite stimulation | |
Wang, Oumei; Zheng, Shiling; Wang, Bingchen; Wang, Wenjing; Liu, Fanghua
| |
| 发表期刊 | PEERJ
 |
| ISSN | 2167-8359 |
| 2018-03-21 | |
| 卷号 | 6页码:e4541 |
| 关键词 | Geobacter metallireducens Methanosarcina barkeri Electrically conductive pili (e-pili) Direct interspecies electron transfer (DIET) Magnetite Ferrous iron Ethanol metabolism Stimulation Co-cultures Methane |
| 研究领域 | Multidisciplinary Sciences |
| DOI | 10.7717/peerj.4541 |
| 产权排序 | [Wang, Oumei] Binzhou Med Univ, Yantai, Shandong, Peoples R China; [Zheng, Shiling; Wang, Bingchen; Wang, Wenjing; Liu, Fanghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Shandong, Peoples R China; [Wang, Bingchen] Univ Chinese Acad Sci, Beijing, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Background: Magnetite-mediated direct interspecies electron transfer (DIET) between Geobacter and Methanosarcina species is increasingly being invoked to explain magnetite stimulation of methane production in anaerobic soils and sediments. Although magnetite-mediated DIET has been documented in defined co-cultures reducing fumarate or nitrate as the electron acceptor, the effects of magnetite have only been inferred in methanogenic systems. Methods: Concentrations of methane and organic acid were analysed with a gas chromatograph and high-performance liquid chromatography, respectively. The concentration of HCl-extractable Fe(II) was determined by the ferrozine method. The association of the defined co-cultures of G. metallireducens and M. barkeri with magnetite was observed with transmission electron micrographs. Results: Magnetite stimulated ethanol metabolism and methane production in defined co-cultures of G. metallireducens and M. barkeri; however, magnetite did not promote methane production in co-cultures initiated with a culture of G. metallireducens that could not produce electrically conductive pili (e-pili), unlike the conductive carbon materials that facilitate DIET in the absence of e-pili. Transmission electron microscopy revealed that G. metallireducens and M. barkeri were closely associated when magnetite was present, as previously observed in G. metallireducens/G. sulfurreducens co-cultures. These results show that magnetite can promote DIET between Geobacter and Methanosarcina species, but not as a substitute for e-pili, and probably functions to facilitate electron transfer from the e-pili to Methanosarcina. Conclusion: In summary, the e-pili are necessary for the stimulation of not only G. metallireducens/G. sulfurreducens, but also methanogenic G. metallireducens/M. barkeri co-cultures with magnetite. |
| 文章类型 | Article |
| 资助机构 | Major Research Plan of the National Natural Science Foundation of China [91751112] ; General Programme of the National Natural Science Foundation of China [41573071, 41371257] ; Key Research Project of Frontier Science of the Chinese Academy of Sciences [QYZDJ-SSW-DQC015] ; Natural Science Foundation of Shandong Province [ZR2016DQ12] ; Young Taishan Scholars Programme [tsqn20161054] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | INTERSPECIES ELECTRON-TRANSFER ; C-TYPE CYTOCHROME ; GEOBACTER-METALLIREDUCENS ; PADDY SOIL ; OXIDE REDUCTION ; FE(III) OXIDE ; SP-NOV ; EXCHANGE ; METHANE ; SULFURREDUCENS |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000428693200003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24383 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Binzhou Med Univ, Yantai, Shandong, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Shandong, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Oumei,Zheng, Shiling,Wang, Bingchen,et al. Necessity of electrically conductive pili for methanogenesis with magnetite stimulation[J]. PEERJ,2018,6:e4541. |
| APA | Wang, Oumei,Zheng, Shiling,Wang, Bingchen,Wang, Wenjing,&Liu, Fanghua.(2018).Necessity of electrically conductive pili for methanogenesis with magnetite stimulation.PEERJ,6,e4541. |
| MLA | Wang, Oumei,et al."Necessity of electrically conductive pili for methanogenesis with magnetite stimulation".PEERJ 6(2018):e4541. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Necessity of electri（18731KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论