Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin
Yan, MY; Qin, S; Li, BF
发表期刊JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY
ISSN1049-8850
2017
卷号26期号:10页码:1166-1174
关键词Purification structural aspect collagen walleye pollock fish skin
研究领域Food Science & Technology
DOI10.1080/10498850.2015.1011797
产权排序[Yan, Mingyan; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Shandong, Peoples R China; [Li, Bafang] Ocean Univ China, Coll Food Sci & Technol, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Type I collagen was extracted from walleye pollock (Theragra chalcogramma) skin and purified by DEAE-52 cellulose chromatography and gel filtration chromatography with Sephacryl S-300 HR. Fourier transform infrared spectroscopy spectra and X-ray diffraction pattern showed the existence of a helical arrangement, with the distance between the molecular chains of 1.18 nm and the unit height, typical of the triple helix, of 0.27 nm. Scanning electron microscopy (SEM) revealed the collagen had a filamentary structure. Peptide mapping, obtained by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), indicated that peptides with the molecular weight of 1,470, 1,565, 1,570, 2,150, and 2,470 Da were the major products of trypsin digestion of pollock skin collagen.
文章类型Article
资助机构National Natural Science Foundation of China [31201455, 41576155]
收录类别SCI
语种英语
关键词[WOS]Purification ; structural aspect ; collagen ; walleye pollock ; fish skin
研究领域[WOS]Food Science & Technology
WOS记录号WOS:000419358200007
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/24366
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Shandong, Peoples R China;
2.Ocean Univ China, Coll Food Sci & Technol, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
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Yan, MY,Qin, S,Li, BF. Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin[J]. JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY,2017,26(10):1166-1174.
APA Yan, MY,Qin, S,&Li, BF.(2017).Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin.JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY,26(10),1166-1174.
MLA Yan, MY,et al."Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin".JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY 26.10(2017):1166-1174.
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