毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素
Alternative TitleDetermination of four phenolic estrogens in water samples by pressure-assisted electrokinetic injection coupled with capillary electrophoresis
高芳芳; 刘军英; 鹿文慧; 李金花; 刘惠涛
Source Publication色谱
ISSN1000-8713
2018-06-08
Volume36Issue:06Pages:573-577
Keyword毛细管电泳 压力辅助电动进样 胶束电动色谱 酚类雌激素 水样
MOST Discipline Catalogue化学
DOI10.3724/SP.J.1123.2018.01011
Contribution Rank(1)烟台大学化学化工学院; (2)中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室
Department海岸带环境过程实验室
AbstractA capillary electrophoresis (CE) method was developed for the simultaneous separation and determination of four phenolic estrogens (PEs), in which pressure-assisted electrokinetic injection (PAEKI) was adopted as the on-line concentration method to enrich the PEs. Several parameters affecting PAEKI conditions such as injection voltage and injection time, were systematically investigated and compared with the usual parameters. Under the optimized PAEKI conditions (-9 kV, 0.3 psi (ca. 2.1 kPa), 0.4 min), the four PEs separated completely within 7 min. Good linearities were attained in the range of 0.05-5 mg/L for hexestrol and dienestrol, and 0.1-10 mg/L for bisphenol A and diethylstilbestrol, with correlation coefficients (r) over 0.9936. The limits of detection (S/N=3) were 0.0071-0.017 mg/L, and enrichment factors ranged from 11 to 15 compared to normal hydrodynamic injection. The combined micellar electrokinetic chromatographic-PAEKI method developed was used to determine the PEs in tap water and lake water samples; limits of quantification (S/N=10) of 0.029-0.064 mg/L and 0.033-0.079 mg/L were attained, respectively, by the two sample types. Furthermore, recoveries ranged from 75.6% to 110.1% with relative standard deviations (n=5) within 4.6%-11.8%. To use this PAEKI method, researchers would only need to adjust the parameters of the CE apparatus to perform on-line enrichment of analytes, without using other reagents; this demonstrates the simplicity, rapidity, and highly automated nature of this method.
Other Abstract在毛细管电泳的胶束电动色谱(MEKC)模式下,采用压力辅助电动进样(PAEKI)的进样方式在线富集4种酚类雌激素(PEs)。对影响PAEKI的进样电压、进样时间等进行考察,并与传统的压力进样比较。结果表明,在最优的PAEKI条件下(-9 k V,0.3 psi(约2.1 k Pa),0.4 min),4种PEs在7 min内基线分离,线性关系良好,相关系数(r)大于0.993 6,己烷雌酚和双烯雌酚的线性范围为0.05~5 mg/L、双酚A和己烯雌酚的线性范围为0.1~10m g/L;检出限(S/N=3)为0.007 1~0.017 m g/L,富集倍数为11~15。使用该MEKC-PAEKI法对自来水和湖水水样进行测定,得到定量限(S/N=10)分别为0.029~0.064 mg/L和0.033~0.079 mg/L;加标回收率为75.6%~110.1%,相对标准偏差(n=5)为4.6%~11.8%。PAEKI不需要使用其他试剂,只需对电泳仪的参数进行适当调整即可实现对分析物的在线富集,简单、快速、自动化程度高。
Indexed ByCSCD
Language中文
Funding ProjectNational Natural Science Foundation of China[21477160] ; National Natural Science Foundation of China[21477160]
CSCD IDCSCD:6275211
Funding Organization国家自然科学基金项目#21477160
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24329
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
Affiliation1.烟台大学化学化工学院;
2.中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室
Recommended Citation
GB/T 7714
高芳芳,刘军英,鹿文慧,等. 毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素[J]. 色谱,2018,36(06):573-577.
APA 高芳芳,刘军英,鹿文慧,李金花,&刘惠涛.(2018).毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素.色谱,36(06),573-577.
MLA 高芳芳,et al."毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素".色谱 36.06(2018):573-577.
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