Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics

doi:10.1016/j.aquatox.2017.09.010

	Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics
	Song, Qinqin 1,2,3; Zhou, Hailong 1,2; Han, Qian 1,2; Diao, Xiaoping 1,2
发表期刊	AQUATIC TOXICOLOGY
ISSN	0166-445X
	2017-11-01
卷号	192 页码:48-57
关键词	Benzo(a)Pyrene Ddt P. Viridis Hepatopancreas Itraq Metabolomics
研究领域	Marine & Freshwater Biology ; Toxicology
DOI	10.1016/j.aquatox.2017.09.010
产权排序	[Song, Qinqin; Zhou, Hailong; Han, Qian; Diao, Xiaoping] Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China; [Song, Qinqin; Zhou, Hailong; Han, Qian; Diao, Xiaoping] Hainan Univ, Inst Trop Agr & Forestry, 58 Renmin Rd, Haikou 570228, Hainan, Peoples R China; [Song, Qinqin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
作者部门	中国科学院海岸带环境过程重点实验室
英文摘要	Dichlorodiphenyltrichloroethane (DDT) and benzo(a)pyrene (BaP) are environmental estrogens (EEs) that are ubiquitous in the marine environment. In the present study, we integrated isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic and nuclear magnetic resonance (NMR)-based metabolomic approaches to explore the toxic responses of green mussel hepatopancreas exposed to DDT (10 mu g/L), BaP (10 mu g/L) and their mixture. The metabolic responses indicated that BaP primarily disturbed energy metabolism and osmotic regulation in the hepatopancreas of the male green mussel P. viridis. Both DDT and the mixture of DDT and BaP perturbed the energy metabolism and osmotic regulation in P. viridis. The proteomic responses revealed that BaP affected the proteins involved in energy metabolism, material transformation, cytoskeleton, stress responses, reproduction and development in green mussels. DDT exposure could change the proteins involved in primary metabolism, stress responses, cytoskeleton and signal transduction. However, the mixture of DDT and BaP altered proteins associated with material and energy metabolism, stress responses, signal transduction, reproduction and development, cytoskeleton and apoptosis. This study showed that iTRAQ-based proteomic and NMR-based metabolomic approaches could effectively elucidate the essential molecular mechanism of disturbances in hepatopancreas function of green mussels exposed to environmental estrogens.
文章类型	Article
资助机构	National Natural Science Foundation of China(41161077, 31760164) ; Specialized Research Fund for the Midwest Program of Hainan University(ZXBJH-XK002)
收录类别	SCI
语种	英语
关键词[WOS]	MUSSEL MYTILUS-GALLOPROVINCIALIS ; CLAM RUDITAPES-PHILIPPINARUM ; EARTHWORM EISENIA-FOETIDA ; OYSTER CRASSOSTREA-GIGAS ; HEAT-SHOCK PROTEINS ; GENDER-SPECIFIC RESPONSES ; 2,2',4,4'-TETRABROMODIPHENYL ETHER ; PETROCHEMICAL CONTAMINATION ; H-1-NMR SPECTROSCOPY ; SALINITY ACCLIMATION
研究领域[WOS]	Marine & Freshwater Biology ; Toxicology
WOS记录号	WOS:000415768700007
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24046
专题	中国科学院烟台海岸带研究所知识产出
通讯作者	Zhou, Hailong
作者单位	1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 2.Hainan Univ, Inst Trop Agr & Forestry, 58 Renmin Rd, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Song, Qinqin,Zhou, Hailong,Han, Qian,et al. Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics[J]. AQUATIC TOXICOLOGY,2017,192:48-57.
APA	Song, Qinqin,Zhou, Hailong,Han, Qian,&Diao, Xiaoping.(2017).Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.AQUATIC TOXICOLOGY,192,48-57.
MLA	Song, Qinqin,et al."Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics".AQUATIC TOXICOLOGY 192(2017):48-57.