Molecular characterization, expression and functional analysis of two Kazal-type serine protease inhibitors from Venerupis philippinarum
Yu, Qian1,3; Yang, Dinglong1,3; Wang, Qing1; Zhang, Yingying1,3; Cong, Ming1; Wu, Huifeng1; Ji, Chenglong1; Li, Fei1; Zhao, Jianmin1,2
2017-11-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号70页码:156-163
摘要Kazal-type serine protease inhibitors (KSPIs) act as negative regulators in immune signaling pathway by controlling the extent of serine protease (SP) activities. In this study, the full-length cDNA of two KSPIs (designed as VpKSPI-1 and VpKSPI-2) were identified from Venerupis philippinarum by rapid amplification of cDNA ends (RACE) approaches. The open reading frame (ORF) of VpKSPI-1 and VpKSPI-2 was of 552 bp and 402 bp, encoding a polypeptide of 183 and 133 amino acids, respectively. The transcripts of VpKSPI-1 and VpKSPI-2 were ubiquitously expressed in all tissues tested with the highest expression level in hepatopancreas. After Vibrio anguillarum challenge, the relative mRNA expression of VpKSPI-1 and VpKSPI-2 in hepatopancreas was both up-regulated within 96 h. The recombinant VpKSPI-1 (rVpKSPI-1) displayed weak activities towards chymotrypsin, moderate inhibitory activity to trypsin, while rVpKSPI-2 showed significant inhibitory activities against chymotrypsin and trypsin. When the molar ratio of rVpKSPI-2 to chymotrypsin and trypsin reached 1:4 and 1:2, the protease activities could be almost entirely inhibited. All these results suggested that both VpKSPI-1 and VpKSPI-2 perhaps play a vital role in the innate immunity of V. philippinarum. (C) 2017 Elsevier Ltd. All rights reserved.
关键词Venerupis Philippinarum Serine Protease Inhibitor Inhibitory Activity Bacterial Challenge Innate Immunity
DOI10.1016/j.fsi.2017.09.018
项目资助者Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702, XDA11020305) ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14, KFZD-SW-106) ; Youth Innovation Promotion Association of CAS(2016196)
收录类别SCI
关键词[WOS]SHRIMP PENAEUS-MONODON ; PROTEINASE-INHIBITOR ; CRASSOSTREA-VIRGINICA ; ERIOCHEIR-SINENSIS ; CLONING ; DOMAIN ; GENE ; PURIFICATION ; HEMOCYTES ; IMMUNITY
文章类型Article
语种英语
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000416186800017
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/24043
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Zhao, Jianmin
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
通讯作者单位中国科学院烟台海岸带研究所
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Yu, Qian,Yang, Dinglong,Wang, Qing,et al. Molecular characterization, expression and functional analysis of two Kazal-type serine protease inhibitors from Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:156-163.
APA Yu, Qian.,Yang, Dinglong.,Wang, Qing.,Zhang, Yingying.,Cong, Ming.,...&Zhao, Jianmin.(2017).Molecular characterization, expression and functional analysis of two Kazal-type serine protease inhibitors from Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,70,156-163.
MLA Yu, Qian,et al."Molecular characterization, expression and functional analysis of two Kazal-type serine protease inhibitors from Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 70(2017):156-163.
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