Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models
Wang, Rui1,2; Han, Xiaoyue1,2; You, Jinmao1,2; Yu, Fabiao1,2; Chen, Lingxin1,2
2018-03-20
发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
卷号90期号:6页码:4054-4061
摘要As new biomarkers, monoamine oxidases (MAOs) play important roles in maintaining the homeostasis of biogenic amines via catalyzing the oxidation of biogenic amines to corresponding aldehydes with the generation of reactive oxygen species (ROS). MAOs have two isoforms, MAO-A and MAO-B. MAO-A is considered to be a major factor of neuropsychiatric and depressive disorders. However, MAO-B is thought to be involved in several neurodegenerative diseases. Therefore, to explore their distinct roles in different diseases, the selective detection of MAOs is essential. Herein, two new types of near-infrared (NIR) fluorescent probes, MitoCy-NH2 and MitoHCy-NH2, are provided for synergistic imaging of MAO-B and its contribution to oxidative stress in cells and in mice aging models. These probes are composed of three moieties: heptamethine cyanine as fluorophore, propanamide as recognition group, and triphenylphosphonium cation as mitochondrial targeting group. The amine oxidation and beta-elimination reaction can lead to obvious fluorescence increase and color changes from green to blue. The probe MitoHCy-NH2 can be used to synergistically detect MAO-B and its contribution to oxidative stress in the replicative senescence model. And the probe MitoCy-NH2 can offer ratiometric near-infrared fluorescence for the selective detection of MAO-B in the H2O2-induced cell aging model and in mice aging models. The results reveal that there are different MAO-B levels in different ages of mice models. MitoCy-NH2 also can evaluate therapeutic effects of pargyline and selegiline in mice models. The desirable analytical behaviors of our probes make them useful chemical tools for the selective detection of MAO-B and its contribution to oxidative stress in biosystems.
DOI10.1021/acs.analchem.7b05297
项目资助者National Nature Science Foundation of China(21775162, 21405172, 41776110, 21575159) ; program of Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for EcoEnvironmental Sciences, CAS(KF2016-22) ; Instrument Developing Project of the Chinese Academy of Sciences(YZ201662)
收录类别SCI
关键词[WOS]LIVING CELLS ; SUPEROXIDE ANION ; FLUOROGENIC PROBE ; REACTIVE OXYGEN ; IN-VIVO ; MAO-B ; DESIGN ; VISUALIZATION ; PLATFORMS ; CATIONS
文章类型Article
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000428219600058
出版者AMER CHEMICAL SOC
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/24031
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Yu, Fabiao; Chen, Lingxin
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn Technol Shandong Pr, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
通讯作者单位中国科学院烟台海岸带研究所
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Wang, Rui,Han, Xiaoyue,You, Jinmao,et al. Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models[J]. ANALYTICAL CHEMISTRY,2018,90(6):4054-4061.
APA Wang, Rui,Han, Xiaoyue,You, Jinmao,Yu, Fabiao,&Chen, Lingxin.(2018).Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models.ANALYTICAL CHEMISTRY,90(6),4054-4061.
MLA Wang, Rui,et al."Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models".ANALYTICAL CHEMISTRY 90.6(2018):4054-4061.
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