YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action
Yang, Dinglong1,2; Zhang, Qianqian1,2; Wang, Qing1,2; Chen, Lizhu3; Liu, Yongliang1; Cong, Ming4; Wu, Huifeng4; Li, Fei4; Ji, Chenglong4; Zhao, Jianmin1,2
2018-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume80Pages:274-280
AbstractDefensins are small cysteine-rich cationic proteins that are ubiquitously present in both vertebrates and invertebrates and constitute the front line of host innate immunity. In the present study, a defensin-like antimicrobial peptide (designed as RpdefB) was identified and characterized from the manila clam Ruditapes philippinarum. The open reading frame of RpdefB encoded a 70-amino acid polypeptide with a calculated molecular mass of 7.5 kDa and isoelectric point of 8.16. Multiple alignments and phylogenetic analysis strongly suggested that RpdefB was a new member of the defensin family. In non-stimulated clams, RpdefB transcripts were constitutively expressed in all five tested tissues, especially in the hepatopancreas. After Vibrio anguillarum challenge, expression of RpdefB mRNA in hemocytes was significantly up-regulated at 6h, 12 h and 72 h. The synthetic peptide RpdefB showed high antibacterial activity against the Gram-negative bacterium Vibrio splendidus. Moreover, membrane integrity analysis revealed that RpdefB increased the membrane permeability of Escherichia coli and then resulted in cell death. Overall, our results suggested that RpdefB played an important role in the elimination of invading bacterium, perhaps through membrane-disruptive activity.
KeywordRuditapes philippinarum Defensin Antimicrobial activity Membrane disruption
DOI10.1016/j.fsi.2018.06.019
Funding OrganizationNational Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Youth Innovation Promotion Association CAS
WOS KeywordALLOMYRINA-DICHOTOMA DEFENSIN ; OYSTER CRASSOSTREA-GIGAS ; MOLECULAR CHARACTERIZATION ; ESCHERICHIA-COLI ; MARINE-INVERTEBRATES ; BIOFILM DEVELOPMENT ; ARTHROPOD DEFENSIN ; GENE-EXPRESSION ; INNATE IMMUNITY ; HOST-DEFENSE
Language英语
Funding ProjectNational Natural Science Foundation of China[41476126] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020305] ; Key Research Program of the Chinese Academy of Sciences[KZZD-EW-14] ; Youth Innovation Promotion Association CAS[2016196]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000440958100030
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24018
Collection中科院海岸带环境过程与生态修复重点实验室
中国科学院牟平海岸带环境综合试验站
Corresponding AuthorCong, Ming; Zhao, Jianmin
Affiliation1.Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264117, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China
3.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Corresponding Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Dinglong,Zhang, Qianqian,Wang, Qing,et al. A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action[J]. FISH & SHELLFISH IMMUNOLOGY,2018,80:274-280.
APA Yang, Dinglong.,Zhang, Qianqian.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2018).A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action.FISH & SHELLFISH IMMUNOLOGY,80,274-280.
MLA Yang, Dinglong,et al."A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action".FISH & SHELLFISH IMMUNOLOGY 80(2018):274-280.
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