A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action
Yang, Dinglong1,2; Zhang, Qianqian1,2; Wang, Qing1,2; Chen, Lizhu3; Liu, Yongliang1; Cong, Ming4; Wu, Huifeng4; Li, Fei4; Ji, Chenglong4; Zhao, Jianmin1,2
2018-09-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号80页码:274-280
摘要Defensins are small cysteine-rich cationic proteins that are ubiquitously present in both vertebrates and invertebrates and constitute the front line of host innate immunity. In the present study, a defensin-like antimicrobial peptide (designed as RpdefB) was identified and characterized from the manila clam Ruditapes philippinarum. The open reading frame of RpdefB encoded a 70-amino acid polypeptide with a calculated molecular mass of 7.5 kDa and isoelectric point of 8.16. Multiple alignments and phylogenetic analysis strongly suggested that RpdefB was a new member of the defensin family. In non-stimulated clams, RpdefB transcripts were constitutively expressed in all five tested tissues, especially in the hepatopancreas. After Vibrio anguillarum challenge, expression of RpdefB mRNA in hemocytes was significantly up-regulated at 6h, 12 h and 72 h. The synthetic peptide RpdefB showed high antibacterial activity against the Gram-negative bacterium Vibrio splendidus. Moreover, membrane integrity analysis revealed that RpdefB increased the membrane permeability of Escherichia coli and then resulted in cell death. Overall, our results suggested that RpdefB played an important role in the elimination of invading bacterium, perhaps through membrane-disruptive activity.
关键词Ruditapes philippinarum Defensin Antimicrobial activity Membrane disruption
DOI10.1016/j.fsi.2018.06.019
项目资助者National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Research Program of the Chinese Academy of Sciences ; Youth Innovation Promotion Association CAS
关键词[WOS]ALLOMYRINA-DICHOTOMA DEFENSIN ; OYSTER CRASSOSTREA-GIGAS ; MOLECULAR CHARACTERIZATION ; ESCHERICHIA-COLI ; MARINE-INVERTEBRATES ; BIOFILM DEVELOPMENT ; ARTHROPOD DEFENSIN ; GENE-EXPRESSION ; INNATE IMMUNITY ; HOST-DEFENSE
语种英语
资助项目National Natural Science Foundation of China[41476126] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020305] ; Key Research Program of the Chinese Academy of Sciences[KZZD-EW-14] ; Youth Innovation Promotion Association CAS[2016196]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000440958100030
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/24018
专题中科院海岸带环境过程与生态修复重点实验室
中国科学院牟平海岸带环境综合试验站
通讯作者Cong, Ming; Zhao, Jianmin
作者单位1.Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264117, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China
3.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
通讯作者单位中国科学院烟台海岸带研究所
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Yang, Dinglong,Zhang, Qianqian,Wang, Qing,et al. A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action[J]. FISH & SHELLFISH IMMUNOLOGY,2018,80:274-280.
APA Yang, Dinglong.,Zhang, Qianqian.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2018).A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action.FISH & SHELLFISH IMMUNOLOGY,80,274-280.
MLA Yang, Dinglong,et al."A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action".FISH & SHELLFISH IMMUNOLOGY 80(2018):274-280.
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