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Coupling between Nitrogen Fixation and Tetrachlorobiphenyl Dechlorination in a Rhizobium-Legume Symbiosis
Wang, Xiaomi1,4; Teng, Ying1; Tu, Chen2; Luo, Yongming1,2; Greening, Chris3; Zhang, Ning1; Dai, Shixiang1,4; Ren, Wenjie1; Zhao, Ling1; Li, Zhengao1

Legume-rhizobium symbioses have the potential to remediate soils contaminated with chlorinated organic compounds. Here, the model symbiosis between Medicago sativa and Sinorhizobium meliloti was used to explore the relationships between symbiotic nitrogen fixation and transformation of tetrachlorobiphenyl PCB 77 within this association. 45-day-old seedlings in vermiculite were pretreated with 5 mg L-1 PCB 77 for 5 days. In PCB-supplemented nodules, addition of the nitrogenase enhancer molybdate significantly stimulated dechlorination by 7.2-fold and reduced tissue accumulation of PCB 77 (roots by 96% and nodules by 93%). Conversely, dechlorination decreased in plants exposed to a nitrogenase inhibitor (nitrate) or harboring nitrogenase-deficient symbionts (nifA mutant) by 29% and 72%, respectively. A range of dechlorinated products (biphenyl, methylbiphenyls, hydroxylbiphenyls, and trichlorobiphenyl derivatives) were detected within nodules and roots under nitrogen-fixing conditions. Levels of nitrogenase-derived hydrogen and leghemoglobin expression correlated positively with nodular dechlorination rates, suggesting a more reducing environment promotes PCB dechlorination. Our findings demonstrate for the first time that symbiotic nitrogen fixation acts as a driving force for tetrachlorobiphenyl dechlorination. In turn, this opens new possibilities for using rhizobia to enhance phytoremediation of halogenated organic compounds.

Funding OrganizationNational Natural Science Foundation of China(41371309, 41671327, 41230858) ; Outstanding Youth Fund of Jiangsu Province(BK20150049) ; ARC DECRA Fellowship(DE170100310)
Indexed BySCI
WOS KeywordZea-mays L. ; Polychlorinated-biphenyls ; Oxidative Stress ; Phanerochaete-chrysosporium ; Sinorhizobium-meliloti ; Medicago-truncatula ; Nitrate Reductase ; Molybdenum ; Alfalfa ; Soils
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS IDWOS:000426143300059
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorTeng, Ying; Luo, Yongming
Affiliation1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Corresponding Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xiaomi,Teng, Ying,Tu, Chen,et al. Coupling between Nitrogen Fixation and Tetrachlorobiphenyl Dechlorination in a Rhizobium-Legume Symbiosis[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2018,52(4):2217-2224.
APA Wang, Xiaomi.,Teng, Ying.,Tu, Chen.,Luo, Yongming.,Greening, Chris.,...&Li, Zhengao.(2018).Coupling between Nitrogen Fixation and Tetrachlorobiphenyl Dechlorination in a Rhizobium-Legume Symbiosis.ENVIRONMENTAL SCIENCE & TECHNOLOGY,52(4),2217-2224.
MLA Wang, Xiaomi,et al."Coupling between Nitrogen Fixation and Tetrachlorobiphenyl Dechlorination in a Rhizobium-Legume Symbiosis".ENVIRONMENTAL SCIENCE & TECHNOLOGY 52.4(2018):2217-2224.
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