A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress

doi:10.1039/c7sc02888a

	A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress
	Han, Xiaoyue 1,2; Song, Xinyu 1; Yu, Fabiao 1; Chen, Lingxin1 ; Yu, Fabiao(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
发表期刊	CHEMICAL SCIENCE
ISSN	2041-6520
	2017-10-01
卷号	8 期号:10 页码:6991-7002
关键词	HIGHLY SELECTIVE DETECTION SE-N BOND GENE-EXPRESSION LIVING CELLS DESIGN STRATEGIES REACTIVE OXYGEN IN-VIVO GLUTATHIONE THIOLS INDUCTION
研究领域	Chemistry, Multidisciplinary
DOI	10.1039/c7sc02888a
产权排序	第1完成单位
作者部门	海岸带环境工程技术研究与发展中心
英文摘要	Hypothermia and hyperthermia are cell stressed states resulting from environmental temperature changes, which can abnormally decrease intracellular glutathione (GSH) concentrations and induce apoptosis. As the most abundant intracellular non-protein biothiol, GSH can protect cells from apoptosis. Considering the important roles of GSH in the anti-apoptotic process in cells and in vivo, we strive to develop a powerful chemical tool for the direct detection of GSH concentration changes under temperature stress. Herein, we report a ratiometric fluorescent probe (CyO-Dise) based on a selenium-sulfur exchange reaction for the qualitative and quantitative detection of GSH concentration fluctuations in cells and in vivo. The probe has been successfully used to assess the changes of GSH levels in HepG2 and HL-7702 cells using the stimulations of hypothermia and hyperthermia. In terms of the anti-apoptotic effect of GSH under hypothermic and hyperthermic conditions, human normal liver HL-7702 cells have stronger abilities to fight against temperature stress than human liver carcinoma HepG2 cells. Hypothermia and hyperthermia can also improve the drug resistance of cis-dichlorodiamineplatinum(II) (DDP)-resistant HepG2/DDP cells. The CyO-Dise probe has been employed to image GSH concentration changes in HepG2 and HepG2/DDP xenografts on nude mice. With the adjuvant therapy effects of hypothermia and hyperthermia, the chemotherapy drug DDP exhibits good ability for the treatment of HepG2 and HepG2/DDP xenografts. The above applications make our probe a potential new candidate for the accurate diagnosis of cancer and efficacy evaluation of treatment.
文章类型	Article
资助机构	National Nature Science Foundation of China(21405172, 21775162, 21575159) ; Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS(KF2016-22) ; Research Center for Eco-Environmental Sciences, CAS(KF2016-22)
收录类别	SCI
语种	英语
关键词[WOS]	HIGHLY SELECTIVE DETECTION ; SE-N BOND ; GENE-EXPRESSION ; LIVING CELLS ; DESIGN STRATEGIES ; REACTIVE OXYGEN ; IN-VIVO ; GLUTATHIONE ; THIOLS ; INDUCTION
研究领域[WOS]	Chemistry
WOS记录号	WOS:000411730500032
引用统计	被引频次：111[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/23454
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Yu, Fabiao; Chen, Lingxin; Yu, Fabiao(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,et al. A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress[J]. CHEMICAL SCIENCE,2017,8(10):6991-7002.
APA	Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,Chen, Lingxin,Yu, Fabiao,&Chen, Lingxin.(2017).A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress.CHEMICAL SCIENCE,8(10),6991-7002.
MLA	Han, Xiaoyue,et al."A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress".CHEMICAL SCIENCE 8.10(2017):6991-7002.