A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress
Han, Xiaoyue1,2; Song, Xinyu1; Yu, Fabiao1; Chen, Lingxin1; Yu, Fabiao(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
2017-10-01
发表期刊CHEMICAL SCIENCE
ISSN2041-6520
卷号8期号:10页码:6991-7002
产权排序第1完成单位
摘要Hypothermia and hyperthermia are cell stressed states resulting from environmental temperature changes, which can abnormally decrease intracellular glutathione (GSH) concentrations and induce apoptosis. As the most abundant intracellular non-protein biothiol, GSH can protect cells from apoptosis. Considering the important roles of GSH in the anti-apoptotic process in cells and in vivo, we strive to develop a powerful chemical tool for the direct detection of GSH concentration changes under temperature stress. Herein, we report a ratiometric fluorescent probe (CyO-Dise) based on a selenium-sulfur exchange reaction for the qualitative and quantitative detection of GSH concentration fluctuations in cells and in vivo. The probe has been successfully used to assess the changes of GSH levels in HepG2 and HL-7702 cells using the stimulations of hypothermia and hyperthermia. In terms of the anti-apoptotic effect of GSH under hypothermic and hyperthermic conditions, human normal liver HL-7702 cells have stronger abilities to fight against temperature stress than human liver carcinoma HepG2 cells. Hypothermia and hyperthermia can also improve the drug resistance of cis-dichlorodiamineplatinum(II) (DDP)-resistant HepG2/DDP cells. The CyO-Dise probe has been employed to image GSH concentration changes in HepG2 and HepG2/DDP xenografts on nude mice. With the adjuvant therapy effects of hypothermia and hyperthermia, the chemotherapy drug DDP exhibits good ability for the treatment of HepG2 and HepG2/DDP xenografts. The above applications make our probe a potential new candidate for the accurate diagnosis of cancer and efficacy evaluation of treatment.
DOI10.1039/c7sc02888a
项目资助者National Nature Science Foundation of China(21405172, 21775162, 21575159) ; Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS(KF2016-22) ; Research Center for Eco-Environmental Sciences, CAS(KF2016-22)
URL查看原文
收录类别SCI
关键词[WOS]HIGHLY SELECTIVE DETECTION ; SE-N BOND ; GENE-EXPRESSION ; LIVING CELLS ; DESIGN STRATEGIES ; REACTIVE OXYGEN ; IN-VIVO ; GLUTATHIONE ; THIOLS ; INDUCTION
文章类型Article
语种英语
WOS研究方向Chemistry
WOS记录号WOS:000411730500032
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/23454
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Yu, Fabiao; Chen, Lingxin; Yu, Fabiao(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
通讯作者单位中国科学院烟台海岸带研究所
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Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,et al. A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress[J]. CHEMICAL SCIENCE,2017,8(10):6991-7002.
APA Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,Chen, Lingxin,Yu, Fabiao,&Chen, Lingxin.(2017).A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress.CHEMICAL SCIENCE,8(10),6991-7002.
MLA Han, Xiaoyue,et al."A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress".CHEMICAL SCIENCE 8.10(2017):6991-7002.
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