Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8
Min, Jun1; Chen, Weiwei1; Wang, Jinpei2; Hu, Xiaoke1; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
2017-09-13
Volume8Pages:1778
Keyword2-chloro-5-nitrophenol Catabolism Cupriavidus Sp Strain Cnp-8 Degradation Kinetics Molecular Mechanism
MOST Discipline CatalogueMicrobiology
DOI10.3389/fmicb.2017.01778
Contribution Rank第1完成单位
Department海岸带生物学与生物资源保护实验室
AbstractCompound 2-chloro-5-nitrophenol (2C5NP) is a typical chlorinated nitroaromatic pollutant. To date, the bacteria with the ability to degrade 2C5NP are rare, and the molecular mechanism of 2C5NP degradation remains unknown. In this study, Cupriavidus sp. strain CNP-8 utilizing 2-chloro-5-nitrophenol (2C5NP) and meta-nitrophenol (MNP) via partial reductive pathways was isolated from pesticide-contaminated soil. Biodegradation kinetic analysis indicated that 2C5NP degradation by this strain was concentration dependent, with a maximum specific degradation rate of 21.2 +/- 2.3 mu M h(-1). Transcriptional analysis showed that the mnp genes are up-regulated in both 2C5NP- and MNP-induced strain CNP-8. Two Mnp proteins were purified to homogeneity by Ni-NTA affinity chromatography. In addition to catalyzing the reduction of MNP, MnpA, a NADPH-dependent nitroreductase, also catalyzes the partial reduction of 2C5NP to 2-chloro-5-hydroxylaminophenol via 2-chloro-5-nitrosophenol, which was firstly identified as an intermediate of 2C5NP catabolism. MnpC, an aminohydroquinone dioxygenase, is likely responsible for the ring-cleavage reaction of 2C5NP degradation. Gene knockout and complementation indicated that mnpA is necessary for both 2C5NP and MNP catabolism. To our knowledge, strain CNP-8 is the second 2C5NP-utilizing bacterium, and this is the first report of the molecular mechanism of microbial 2C5NP degradation.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordRALSTONIA-EUTROPHA JMP134 ; PSEUDOMONAS SP JHN ; PARA-NITROPHENOL ; 2-CHLORO-4-NITROPHENOL DEGRADATION ; SPHINGOMONAS-PAUCIMOBILIS ; CLEAVAGE DIOXYGENASE ; AEROBIC DEGRADATION ; CATABOLISM PATHWAY ; DIETHYL PHTHALATE ; CONTAMINATED SOIL
WOS Research AreaMicrobiology
WOS IDWOS:000410482100001
Funding OrganizationNational Natural Science Foundation of China(31600085) ; National Key Research Program of China(2016YFC1402300) ; Foreword Key Priority Research Program of Chinese Academy of Sciences(QYZDB-SSW-DQC013) ; State Key Laboratory of Microbial Metabolism, Shanghai Tong University(MMLKF15-04)
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/23422
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Corresponding AuthorHu, Xiaoke; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Min, Jun,Chen, Weiwei,Wang, Jinpei,et al. Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8[J]. FRONTIERS IN MICROBIOLOGY,2017,8:1778.
APA Min, Jun,Chen, Weiwei,Wang, Jinpei,Hu, Xiaoke,&Hu, Xiaoke.(2017).Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8.FRONTIERS IN MICROBIOLOGY,8,1778.
MLA Min, Jun,et al."Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8".FRONTIERS IN MICROBIOLOGY 8(2017):1778.
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