| Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8 | |
Min, Jun1 ; Chen, Weiwei1; Wang, Jinpei2; Hu, Xiaoke1; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
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| Source Publication | FRONTIERS IN MICROBIOLOGY
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| ISSN | 1664-302X |
| 2017-09-13 | |
| Volume | 8Pages:1778 |
| Keyword | 2-chloro-5-nitrophenol Catabolism Cupriavidus Sp Strain Cnp-8 Degradation Kinetics Molecular Mechanism |
| MOST Discipline Catalogue | Microbiology |
| DOI | 10.3389/fmicb.2017.01778 |
| Contribution Rank | 第1完成单位 |
| Department | 海岸带生物学与生物资源保护实验室 |
| Abstract | Compound 2-chloro-5-nitrophenol (2C5NP) is a typical chlorinated nitroaromatic pollutant. To date, the bacteria with the ability to degrade 2C5NP are rare, and the molecular mechanism of 2C5NP degradation remains unknown. In this study, Cupriavidus sp. strain CNP-8 utilizing 2-chloro-5-nitrophenol (2C5NP) and meta-nitrophenol (MNP) via partial reductive pathways was isolated from pesticide-contaminated soil. Biodegradation kinetic analysis indicated that 2C5NP degradation by this strain was concentration dependent, with a maximum specific degradation rate of 21.2 +/- 2.3 mu M h(-1). Transcriptional analysis showed that the mnp genes are up-regulated in both 2C5NP- and MNP-induced strain CNP-8. Two Mnp proteins were purified to homogeneity by Ni-NTA affinity chromatography. In addition to catalyzing the reduction of MNP, MnpA, a NADPH-dependent nitroreductase, also catalyzes the partial reduction of 2C5NP to 2-chloro-5-hydroxylaminophenol via 2-chloro-5-nitrosophenol, which was firstly identified as an intermediate of 2C5NP catabolism. MnpC, an aminohydroquinone dioxygenase, is likely responsible for the ring-cleavage reaction of 2C5NP degradation. Gene knockout and complementation indicated that mnpA is necessary for both 2C5NP and MNP catabolism. To our knowledge, strain CNP-8 is the second 2C5NP-utilizing bacterium, and this is the first report of the molecular mechanism of microbial 2C5NP degradation. |
| Subtype | Article |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | RALSTONIA-EUTROPHA JMP134 ; PSEUDOMONAS SP JHN ; PARA-NITROPHENOL ; 2-CHLORO-4-NITROPHENOL DEGRADATION ; SPHINGOMONAS-PAUCIMOBILIS ; CLEAVAGE DIOXYGENASE ; AEROBIC DEGRADATION ; CATABOLISM PATHWAY ; DIETHYL PHTHALATE ; CONTAMINATED SOIL |
| WOS Research Area | Microbiology |
| WOS ID | WOS:000410482100001 |
| Funding Organization | National Natural Science Foundation of China(31600085) ; National Key Research Program of China(2016YFC1402300) ; Foreword Key Priority Research Program of Chinese Academy of Sciences(QYZDB-SSW-DQC013) ; State Key Laboratory of Microbial Metabolism, Shanghai Tong University(MMLKF15-04) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/23422 |
| Collection | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| Corresponding Author | Hu, Xiaoke; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China) |
| Affiliation | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Min, Jun,Chen, Weiwei,Wang, Jinpei,et al. Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8[J]. FRONTIERS IN MICROBIOLOGY,2017,8:1778. |
| APA | Min, Jun,Chen, Weiwei,Wang, Jinpei,Hu, Xiaoke,&Hu, Xiaoke.(2017).Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8.FRONTIERS IN MICROBIOLOGY,8,1778. |
| MLA | Min, Jun,et al."Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8".FRONTIERS IN MICROBIOLOGY 8(2017):1778. |
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