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题名:
Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8
作者: Min, Jun1; Chen, Weiwei1; Wang, Jinpei2; Hu, Xiaoke1
刊名: FRONTIERS IN MICROBIOLOGY
ISSN号: 1664-302X
出版日期: 2017-09-13
卷号: 8
关键词: 2-chloro-5-nitrophenol ; catabolism ; Cupriavidus sp strain CNP-8 ; degradation kinetics ; molecular mechanism
DOI: 10.3389/fmicb.2017.01778
产权排序: 第1完成单位
通讯作者: Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
英文摘要: Compound 2-chloro-5-nitrophenol (2C5NP) is a typical chlorinated nitroaromatic pollutant. To date, the bacteria with the ability to degrade 2C5NP are rare, and the molecular mechanism of 2C5NP degradation remains unknown. In this study, Cupriavidus sp. strain CNP-8 utilizing 2-chloro-5-nitrophenol (2C5NP) and meta-nitrophenol (MNP) via partial reductive pathways was isolated from pesticide-contaminated soil. Biodegradation kinetic analysis indicated that 2C5NP degradation by this strain was concentration dependent, with a maximum specific degradation rate of 21.2 +/- 2.3 mu M h(-1). Transcriptional analysis showed that the mnp genes are up-regulated in both 2C5NP- and MNP-induced strain CNP-8. Two Mnp proteins were purified to homogeneity by Ni-NTA affinity chromatography. In addition to catalyzing the reduction of MNP, MnpA, a NADPH-dependent nitroreductase, also catalyzes the partial reduction of 2C5NP to 2-chloro-5-hydroxylaminophenol via 2-chloro-5-nitrosophenol, which was firstly identified as an intermediate of 2C5NP catabolism. MnpC, an aminohydroquinone dioxygenase, is likely responsible for the ring-cleavage reaction of 2C5NP degradation. Gene knockout and complementation indicated that mnpA is necessary for both 2C5NP and MNP catabolism. To our knowledge, strain CNP-8 is the second 2C5NP-utilizing bacterium, and this is the first report of the molecular mechanism of microbial 2C5NP degradation.
研究领域[WOS]: Microbiology
项目资助者: National Natural Science Foundation of China(31600085) ; National Key Research Program of China(2016YFC1402300) ; Foreword Key Priority Research Program of Chinese Academy of Sciences(QYZDB-SSW-DQC013) ; State Key Laboratory of Microbial Metabolism, Shanghai Tong University(MMLKF15-04)
关键词[WOS]: RALSTONIA-EUTROPHA JMP134 ; PSEUDOMONAS SP JHN ; PARA-NITROPHENOL ; 2-CHLORO-4-NITROPHENOL DEGRADATION ; SPHINGOMONAS-PAUCIMOBILIS ; CLEAVAGE DIOXYGENASE ; AEROBIC DEGRADATION ; CATABOLISM PATHWAY ; DIETHYL PHTHALATE ; CONTAMINATED SOIL
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000410482100001
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内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/23422
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan, Hubei, Peoples R China

Recommended Citation:
Min, Jun,Chen, Weiwei,Wang, Jinpei,et al. Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
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