Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8
Min, Jun1; Chen, Weiwei1; Wang, Jinpei2; Hu, Xiaoke1; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
2017-09-13
发表期刊FRONTIERS IN MICROBIOLOGY
ISSN1664-302X
卷号8
产权排序第1完成单位
摘要Compound 2-chloro-5-nitrophenol (2C5NP) is a typical chlorinated nitroaromatic pollutant. To date, the bacteria with the ability to degrade 2C5NP are rare, and the molecular mechanism of 2C5NP degradation remains unknown. In this study, Cupriavidus sp. strain CNP-8 utilizing 2-chloro-5-nitrophenol (2C5NP) and meta-nitrophenol (MNP) via partial reductive pathways was isolated from pesticide-contaminated soil. Biodegradation kinetic analysis indicated that 2C5NP degradation by this strain was concentration dependent, with a maximum specific degradation rate of 21.2 +/- 2.3 mu M h(-1). Transcriptional analysis showed that the mnp genes are up-regulated in both 2C5NP- and MNP-induced strain CNP-8. Two Mnp proteins were purified to homogeneity by Ni-NTA affinity chromatography. In addition to catalyzing the reduction of MNP, MnpA, a NADPH-dependent nitroreductase, also catalyzes the partial reduction of 2C5NP to 2-chloro-5-hydroxylaminophenol via 2-chloro-5-nitrosophenol, which was firstly identified as an intermediate of 2C5NP catabolism. MnpC, an aminohydroquinone dioxygenase, is likely responsible for the ring-cleavage reaction of 2C5NP degradation. Gene knockout and complementation indicated that mnpA is necessary for both 2C5NP and MNP catabolism. To our knowledge, strain CNP-8 is the second 2C5NP-utilizing bacterium, and this is the first report of the molecular mechanism of microbial 2C5NP degradation.
关键词2-chloro-5-nitrophenol Catabolism Cupriavidus Sp Strain Cnp-8 Degradation Kinetics Molecular Mechanism
DOI10.3389/fmicb.2017.01778
项目资助者National Natural Science Foundation of China(31600085) ; National Key Research Program of China(2016YFC1402300) ; Foreword Key Priority Research Program of Chinese Academy of Sciences(QYZDB-SSW-DQC013) ; State Key Laboratory of Microbial Metabolism, Shanghai Tong University(MMLKF15-04)
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收录类别SCI
关键词[WOS]RALSTONIA-EUTROPHA JMP134 ; PSEUDOMONAS SP JHN ; PARA-NITROPHENOL ; 2-CHLORO-4-NITROPHENOL DEGRADATION ; SPHINGOMONAS-PAUCIMOBILIS ; CLEAVAGE DIOXYGENASE ; AEROBIC DEGRADATION ; CATABOLISM PATHWAY ; DIETHYL PHTHALATE ; CONTAMINATED SOIL
文章类型Article
语种英语
WOS研究方向Microbiology
WOS记录号WOS:000410482100001
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文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/23422
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Hu, Xiaoke; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan, Hubei, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
通讯作者单位中国科学院烟台海岸带研究所
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Min, Jun,Chen, Weiwei,Wang, Jinpei,et al. Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
APA Min, Jun,Chen, Weiwei,Wang, Jinpei,Hu, Xiaoke,&Hu, Xiaoke.(2017).Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8.FRONTIERS IN MICROBIOLOGY,8.
MLA Min, Jun,et al."Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8".FRONTIERS IN MICROBIOLOGY 8(2017).
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