Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems
Liu, Wei1; Yang, Jinbao2; Sun, Yu1,3; Liu, Xiaolin1,3; Li, Yan1; Zhang, Zhenpeng1,3; Xie, Zhihong1; Xie, Zhihong(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
2017-07-13
发表期刊FRONTIERS IN MICROBIOLOGY
ISSN1664-302X
卷号8
产权排序第1完成单位
摘要Azorhizobium caulinodans ORS571 is a motile soil bacterium that interacts symbiotically with legume host Sesbania rostrata, forming nitrogen-fixing root and stem nodules. Bacterial chemotaxis plays an important role in establishing this symbiotic relationship. To determine the contribution of chemotaxis to symbiosis in A. caulinodans ORS571-S. rostrata, we characterized the function of TlpA1 (transducer-like protein in A. caulinodans), a chemoreceptor predicted by SMART (Simple Modular Architecture Research Tool), containing two N-terminal transmembrane regions. The tlpA1 gene is located immediately upstream of the unique che gene cluster and is transcriptionally co-oriented. We found that a Delta tlpA1 mutant is severely impaired for chemotaxis to various organic acids, glycerol and proline. Furthermore, biofilm forming ability of the strain carrying the mutation is reduced under certain growth conditions. Interestingly, competitive colonization ability on S. rostrata root surfaces is impaired in the Delta tlpA1 mutant, suggesting that chemotaxis of the A. caulinodans ORS571 contributes to root colonization. We also found that TlpA1 promotes competitive nodulation not only on roots but also on stems of S. rostrata. Taken together, our data strongly suggest that TlpA1 is a transmembrane chemoreceptor involved in A. caulinodans-S. rostrata symbiosis.
关键词Chemotaxis Azorhizobium Caulinodans Chemoreceptor Symbiosis Nodulation
DOI10.3389/fmicb.2017.01327
项目资助者National Natural Science Foundation of China(31370108 ; Chinese Academy of Sciences(KZZD-EW-14) ; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS) ; Shandong Independent Innovation and Achievement Transformation Pro-gram(2014ZZCX07303) ; Shandong Academy of Agricultural Sciences(+CXGC2016B10) ; Shandong Province Science Foundation(ZR2016CB44) ; 31570063)
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收录类别SCI
关键词[WOS]PROTEIN-SEQUENCE ANALYSES ; RHIZOBIUM-MELILOTI ; SESBANIA-ROSTRATA ; BIOFILM FORMATION ; BACTERIAL CHEMORECEPTORS ; AZOSPIRILLUM-BRASILENSE ; CHEMOTAXIS ; MOTILITY ; SYSTEM ; NODULES
文章类型Article
语种英语
WOS研究方向Microbiology
WOS记录号WOS:000406156300001
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文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22568
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Xie, Zhihong; Xie, Zhihong(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China
2.Shanxi Agr Univ, Coll Life Sci, Taigu, Peoples R China
3.Univ Chinese Acad Sci, Sch Resource & Environm, Beijing, Peoples R China
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Liu, Wei,Yang, Jinbao,Sun, Yu,et al. Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
APA Liu, Wei.,Yang, Jinbao.,Sun, Yu.,Liu, Xiaolin.,Li, Yan.,...&Xie, Zhihong.(2017).Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems.FRONTIERS IN MICROBIOLOGY,8.
MLA Liu, Wei,et al."Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems".FRONTIERS IN MICROBIOLOGY 8(2017).
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