YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum
Yang, Dinglong1,3; Wang, Qing1; Chen, Lizhu1,3; Liu, Yongliang1,2; Cao, Ruiwen1,3; Wu, Huifeng1; Li, Fei1; Ji, Chenglong1; Cong, Ming1; Zhao, Jianmin1,2
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2017-06-01
Volume65Pages:17-24
KeywordRuditapes Philippinarum Phage-type Lysozyme Tissue Distribution Antimicrobial Activity
MOST Discipline CatalogueFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2017.03.051
Contribution Rank[Yang, Dinglong; Wang, Qing; Chen, Lizhu; Liu, Yongliang; Cao, Ruiwen; Wu, Huifeng; Li, Fei; Ji, Chenglong; Cong, Ming; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Liu, Yongliang; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Chen, Lizhu; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department海岸带生物资源高效利用研究与发展中心
AbstractA phage-type lysozyme, designed as RpPLYZ, was cloned and characterized from the clam Ruditapes philippinarum. The full-length cDNA of RpPLYZ was of 699 bp with an open reading frame (ORF) of 534 bp, encoding a polypeptide of 177-amino acid with a calculated molecular mass of 19.6 kDa and an isoelectric point of 9.05. Multiple alignments and phylogenetic analysis strongly suggested that RpPLYZ was a new member of the phage-type lysozyme family. The mRNA transcript of RpPLYZ was found to be constitutively expressed in a wide range of tissues and mainly in hemocytes and mantle. The relative expression of RpPLYZ mRNA in hemocytes was significantly up-regulated at 6, 24, 48 and 72 h after Vibrio anguillarum challenge. The recombinant RpPLYZ (rRpPLYZ) showed high activity against Entherobacter cloacae and Staphyloccocus aureus, and less effective towards Entherobacter aerogenes and V. anguillarum. Moreover, the optimal pH, temperature and ionic strength for rRpPLYZ activity was determined to be 5.5, 50 degrees C and 5 mM, respectively. These results suggested that RpPLYZ was a member of the phage-type lysozyme family and perhaps played an important role in the immune responses against bacterial invasion. (C) 2017 Elsevier Ltd. All rights reserved.
Funding OrganizationNational Natural Science Foundation of China(41476126) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702 ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14) ; Youth Innovation Promotion Association, CAS(2016196) ; XDA11020305)
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordINVERTEBRATE-TYPE LYSOZYME ; I-TYPE LYSOZYMES ; ANTIMICROBIAL ACTIVITY ; CRASSOSTREA-VIRGINICA ; EXPRESSION ANALYSIS ; PENAEUS-MONODON ; EASTERN OYSTER ; CDNA CLONING ; DISK ABALONE ; TIGER SHRIMP
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000402943200003
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/22526
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang, Dinglong,Wang, Qing,Chen, Lizhu,et al. Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2017,65:17-24.
APA Yang, Dinglong.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,Cao, Ruiwen.,...&Zhao, Jianmin.(2017).Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,65,17-24.
MLA Yang, Dinglong,et al."Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 65(2017):17-24.
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