Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum

doi:10.1016/j.fsi.2017.03.051

	Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum
	Yang, Dinglong1,3 ; Wang, Qing1 ; Chen, Lizhu 1,3; Liu, Yongliang 1,2; Cao, Ruiwen 1,3; Wu, Huifeng1 ; Li, Fei1 ; Ji, Chenglong1 ; Cong, Ming1 ; Zhao, Jianmin1,2
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2017-06-01
卷号	65 页码:17-24
关键词	Ruditapes Philippinarum Phage-type Lysozyme Tissue Distribution Antimicrobial Activity
研究领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2017.03.051
产权排序	[Yang, Dinglong; Wang, Qing; Chen, Lizhu; Liu, Yongliang; Cao, Ruiwen; Wu, Huifeng; Li, Fei; Ji, Chenglong; Cong, Ming; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Liu, Yongliang; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Chen, Lizhu; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	海岸带生物资源高效利用研究与发展中心
英文摘要	A phage-type lysozyme, designed as RpPLYZ, was cloned and characterized from the clam Ruditapes philippinarum. The full-length cDNA of RpPLYZ was of 699 bp with an open reading frame (ORF) of 534 bp, encoding a polypeptide of 177-amino acid with a calculated molecular mass of 19.6 kDa and an isoelectric point of 9.05. Multiple alignments and phylogenetic analysis strongly suggested that RpPLYZ was a new member of the phage-type lysozyme family. The mRNA transcript of RpPLYZ was found to be constitutively expressed in a wide range of tissues and mainly in hemocytes and mantle. The relative expression of RpPLYZ mRNA in hemocytes was significantly up-regulated at 6, 24, 48 and 72 h after Vibrio anguillarum challenge. The recombinant RpPLYZ (rRpPLYZ) showed high activity against Entherobacter cloacae and Staphyloccocus aureus, and less effective towards Entherobacter aerogenes and V. anguillarum. Moreover, the optimal pH, temperature and ionic strength for rRpPLYZ activity was determined to be 5.5, 50 degrees C and 5 mM, respectively. These results suggested that RpPLYZ was a member of the phage-type lysozyme family and perhaps played an important role in the immune responses against bacterial invasion. (C) 2017 Elsevier Ltd. All rights reserved.
文章类型	Article
资助机构	National Natural Science Foundation of China(41476126) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702 ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14) ; Youth Innovation Promotion Association, CAS(2016196) ; XDA11020305)
收录类别	SCI
语种	英语
关键词[WOS]	INVERTEBRATE-TYPE LYSOZYME ; I-TYPE LYSOZYMES ; ANTIMICROBIAL ACTIVITY ; CRASSOSTREA-VIRGINICA ; EXPRESSION ANALYSIS ; PENAEUS-MONODON ; EASTERN OYSTER ; CDNA CLONING ; DISK ABALONE ; TIGER SHRIMP
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000402943200003
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/22526
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Dinglong,Wang, Qing,Chen, Lizhu,et al. Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2017,65:17-24.
APA	Yang, Dinglong.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,Cao, Ruiwen.,...&Zhao, Jianmin.(2017).Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,65,17-24.
MLA	Yang, Dinglong,et al."Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 65(2017):17-24.