Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum
Yang, Dinglong1,3; Wang, Qing1; Chen, Lizhu1,3; Liu, Yongliang1,2; Cao, Ruiwen1,3; Wu, Huifeng1; Li, Fei1; Ji, Chenglong1; Cong, Ming1; Zhao, Jianmin1,2
2017-06-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号65页码:17-24
摘要A phage-type lysozyme, designed as RpPLYZ, was cloned and characterized from the clam Ruditapes philippinarum. The full-length cDNA of RpPLYZ was of 699 bp with an open reading frame (ORF) of 534 bp, encoding a polypeptide of 177-amino acid with a calculated molecular mass of 19.6 kDa and an isoelectric point of 9.05. Multiple alignments and phylogenetic analysis strongly suggested that RpPLYZ was a new member of the phage-type lysozyme family. The mRNA transcript of RpPLYZ was found to be constitutively expressed in a wide range of tissues and mainly in hemocytes and mantle. The relative expression of RpPLYZ mRNA in hemocytes was significantly up-regulated at 6, 24, 48 and 72 h after Vibrio anguillarum challenge. The recombinant RpPLYZ (rRpPLYZ) showed high activity against Entherobacter cloacae and Staphyloccocus aureus, and less effective towards Entherobacter aerogenes and V. anguillarum. Moreover, the optimal pH, temperature and ionic strength for rRpPLYZ activity was determined to be 5.5, 50 degrees C and 5 mM, respectively. These results suggested that RpPLYZ was a member of the phage-type lysozyme family and perhaps played an important role in the immune responses against bacterial invasion. (C) 2017 Elsevier Ltd. All rights reserved.
关键词Ruditapes Philippinarum Phage-type Lysozyme Tissue Distribution Antimicrobial Activity
DOI10.1016/j.fsi.2017.03.051
项目资助者National Natural Science Foundation of China(41476126) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702 ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14) ; Youth Innovation Promotion Association, CAS(2016196) ; XDA11020305)
收录类别SCI
关键词[WOS]INVERTEBRATE-TYPE LYSOZYME ; I-TYPE LYSOZYMES ; ANTIMICROBIAL ACTIVITY ; CRASSOSTREA-VIRGINICA ; EXPRESSION ANALYSIS ; PENAEUS-MONODON ; EASTERN OYSTER ; CDNA CLONING ; DISK ABALONE ; TIGER SHRIMP
文章类型Article
语种英语
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000402943200003
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22526
专题中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Yang, Dinglong,Wang, Qing,Chen, Lizhu,et al. Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2017,65:17-24.
APA Yang, Dinglong.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,Cao, Ruiwen.,...&Zhao, Jianmin.(2017).Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,65,17-24.
MLA Yang, Dinglong,et al."Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 65(2017):17-24.
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