Photobacterium proteolyticum sp nov., a protease-producing bacterium isolated from ocean sediments of Laizhou Bay
Li, Yan1; Zhou, Mingyang2; Wang, Fengqing3; Wang, En Tao4; Du, Zongjun3; Wu, Chaoya1; Zhang, Zhenpeng1; Liu, Wei1; Xie, Zhihong1
2017-06-01
发表期刊INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN1466-5026
卷号67期号:6页码:1835-1840
摘要A protease-producing bacterial strain, 13-12(T), was isolated from the ocean sediment of Laizhou Bay, PR China and systematically studied. The bacterium was Gram-stain negative, non spore-forming rods, which were motile with two flagella. It was positive for oxidase, the hydrolysis of starch, agar and gelatin, and for nitrate reduction. It was negative for catalase, esterase and the degradation of CM-cellulose. Optimum growth was observed at 28 degrees C, pH 6.5-7.0 and in the presence of 2-3% (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene, and whole genome data, affiliated it to the genus Photobacterium. It was most closely related to Photobacterium jeanii R-40508T (96.7% 16S rRNA gene similarity). Strain 1312(T) was found to have less than 86.1% similarities with the type strains of its most closely related species in multi-locus sequence analysis, less than 75.2% using genome average nucleotide identities (ANI), and less than 18.5% in DNA-DNA relatedness studies. Q8 was the predominant respiratory menaquinone. Phosphatidylethanolamine, phosphoaminolipid and phospholipid were the major polar phospholipids and summed feature 3 (48.2 %), C-16 : 0 (18.4 %) and C-18 : 1 omega 5c (14.1 %) the major fatty acids. The combined phenotypic, phylogenetic, genomic and chemotaxonomic data support this strain representing a novel species of the genus Photobacterium, for which the name Photobacterium proteolyticum sp. nov. is proposed, with 13-12(T) (= KCTC 42764(T) = CGMCC 1.14970) as the type strain. The genome size of 13-12(T) is 6.2 Mbp, comprising 5806 predicted genes and the DNA G+C content is 47.9 mol%.
关键词Marine Bacterium Sediment Polyphasic Taxonomy Genome Phylogeny
DOI10.1099/ijsem.0.001873
项目资助者Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020403) ; National Natural Science Foundation of China(31600009 ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14) ; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS) ; Shandong Independent Innovation and Achievement Transformation Program(2014ZZCX07303) ; IPN, Mexico(SIP 20150597 ; 31370108 ; 20160883) ; 31570063)
收录类别SCI
文章类型Article
语种英语
WOS研究方向Microbiology
WOS记录号WOS:000405503800035
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22519
专题海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China
2.Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan 250353, Peoples R China
3.Shandong Univ, Coll Marine Sci, Weihai 264209, Peoples R China
4.Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City 11340, DF, Mexico
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Li, Yan,Zhou, Mingyang,Wang, Fengqing,et al. Photobacterium proteolyticum sp nov., a protease-producing bacterium isolated from ocean sediments of Laizhou Bay[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2017,67(6):1835-1840.
APA Li, Yan.,Zhou, Mingyang.,Wang, Fengqing.,Wang, En Tao.,Du, Zongjun.,...&Xie, Zhihong.(2017).Photobacterium proteolyticum sp nov., a protease-producing bacterium isolated from ocean sediments of Laizhou Bay.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,67(6),1835-1840.
MLA Li, Yan,et al."Photobacterium proteolyticum sp nov., a protease-producing bacterium isolated from ocean sediments of Laizhou Bay".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 67.6(2017):1835-1840.
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