Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments
Chen, Yulin1,2; Liu, Shiliang A.3; Mou, Haijin2; Ma, Yunxiao2; Li, Meng1; Hu, Xiaoke1
2017-05-16
发表期刊FRONTIERS IN MICROBIOLOGY
ISSN1664-302X
卷号8
摘要Antibiotic resistance has become one of the world's most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptides. A functional bacteria MB01 producing lipopeptides which can be used as bacteriostat was isolated from the Bohai Sea sediments, which had been identified as Bacillus licheniformis by the morphological, physiological, and biochemical identification and 16s rDNA sequence. The lipopeptides produced by MB01 were determined to be cyclic surfactin homologs by LC-ESI-MS structural identification after crude extraction and LH-20 chromatography. [M + H](+) m/z 994, 1008, 1022, and 1036 were all the characteristic molecular weight of surfactin homologs. CID analysis revealed that the molecular structure of the lipopeptides was R-n-Glu(1)-Leu/lle(2)-Leu(3)-Val(4)-Asp(5)-Leu(6)-Leu/Ile(7). The lipopeptides showed well resistance to UV light and the change of pH and temperature.
关键词Bacillus Licheniformis Purification Bacteriostat Structure Lc-esi-ms/ms Stabilization
DOI10.3389/fmicb.2017.00871
项目资助者National Natural Science Foundation of China(41376138 ; North China Sea Branch of State Oceanic Administration(QDZC20150420-002) ; 41576165)
收录类别SCI
关键词[WOS]ANTIMICROBIAL PEPTIDES ; CYCLIC LIPOPEPTIDES ; VIBRIO-PARAHAEMOLYTICUS ; MS/MS CHARACTERIZATION ; MICROBIAL SURFACTANTS ; MASS-SPECTROMETRY ; IDENTIFICATION ; PURIFICATION ; SUBTILIS ; STRAINS
文章类型Article
语种英语
WOS研究方向Microbiology
WOS记录号WOS:000401575500003
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文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22514
专题海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
2.Ocean Univ China, Sch Food Sci & Engn, Qingdao, Peoples R China
3.Louisiana State Univ, Sch Vet Med, Baton Rouge, LA 70803 USA
第一作者单位中国科学院烟台海岸带研究所
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Chen, Yulin,Liu, Shiliang A.,Mou, Haijin,et al. Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
APA Chen, Yulin,Liu, Shiliang A.,Mou, Haijin,Ma, Yunxiao,Li, Meng,&Hu, Xiaoke.(2017).Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments.FRONTIERS IN MICROBIOLOGY,8.
MLA Chen, Yulin,et al."Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments".FRONTIERS IN MICROBIOLOGY 8(2017).
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