Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Characteristics of cadmium uptake and membrane transport in roots of intact wheat (Triticum aestivum L.) seedlings | |
Li, Lian-Zhen ; Tu, Chen; Peijnenburg, Willie J. G. M.; Luo, Yong-Ming; Luo, YM (reprint author), 17 Chunhui Rd, Yantai 264003, Peoples R China. lianzhen.li@yic.ac.cn
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| 发表期刊 | ENVIRONMENTAL POLLUTION
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| ISSN | 0269-7491 |
| 2017-02 | |
| 卷号 | 221页码:351-358 |
| 关键词 | Ion-selective Microelectrodes Heavy-metal Detoxification Durum-wheat Cd Accumulation Corn Roots Fluxes Calcium Ca2++ Plants K++ |
| DOI | 10.1016/j.envpol.2016.11.085 |
| 产权排序 | [Li, Lian-Zhen; Tu, Chen; Luo, Yong-Ming] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai, Peoples R China; [Peijnenburg, Willie J. G. M.] Natl Inst Publ Hlth & Environm, Ctr Safety Subst & Prod, POB 1, NL-3720 BA Bilthoven, Netherlands; [Peijnenburg, Willie J. G. M.] Leiden Univ, Inst Environm Sci CML, Leiden, Netherlands |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Wheat is one of several cereals that is capable of accumulating higher amounts of Cd in plant tissues. It is important to understand the Cd2+ transport processes in roots that result in excess Cd accumulation. Traditional destructive technologies have limited capabilities in analyzing root samples due to methodological limitations, and sometimes may result in false conclusions. The mechanisms of Cd2+ uptake into the roots of wheat seedlings (Triticum aestivum L.) were investigated by assessing the impact of various inhibitors and channel blockers on Cd accumulation as well as the real-time net Cd2+ flux at roots with the non-destructive scanning ion-selective electrode technique. The P-type ATPase inhibitor Na3VO4 (500 mu M) had little effect on Cd uptake (p < 0.05) and the kinetics of transport in the root of wheat, suggesting that Cd2+ uptake into wheat root cells is not directly dependent on H+ gradients. While, the uncoupler 2,4-dinitrophenol significantly limited Cd2+ uptake (p < 0.05) and transport kinetics in the root of wheat, suggesting the existence of metabolic mediation in the Cd2+ uptake process by wheat. The Cd content at the whole-plant level in wheat was significantly (p < 0.05) decreased upon pretreatment with the Ca2+ channel blockers La3+ or Gd3+ and Verapamil, but not in case of pretreatment with the K+ channel blocker tetraethylammonium (TEA). In addition, the inhibitors of the Ca2+ channel, as well as high concentrations of Ca2+, reduced the real-time net Cd2+ fluxes at the root surface in SIET experiments. These results indicate that Cd2+ moves across the plasma lemma of the wheat root via Ca2+ channels. In addition, our results suggested a role for protein synthesis in mediating Cd2+ uptake and transport by wheat. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation [Y311111031, 41230858] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ION-SELECTIVE MICROELECTRODES ; HEAVY-METAL DETOXIFICATION ; DURUM-WHEAT ; CD ACCUMULATION ; CORN ROOTS ; FLUXES ; CALCIUM ; CA2+ ; PLANTS ; K+ |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| WOS记录号 | WOS:000392767900036 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/22073 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Luo, YM (reprint author), 17 Chunhui Rd, Yantai 264003, Peoples R China. lianzhen.li@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai, Peoples R China 2.Natl Inst Publ Hlth & Environm, Ctr Safety Subst & Prod, POB 1, NL-3720 BA Bilthoven, Netherlands 3.Natl Inst Publ Hlth & Environm, Ctr Safety Subst & Prod, POB 1, NL-3720 BA Bilthoven, Netherlands 4.Leiden Univ, Inst Environm Sci CML, Leiden, Netherlands |
| 推荐引用方式 GB/T 7714 | Li, Lian-Zhen,Tu, Chen,Peijnenburg, Willie J. G. M.,et al. Characteristics of cadmium uptake and membrane transport in roots of intact wheat (Triticum aestivum L.) seedlings[J]. ENVIRONMENTAL POLLUTION,2017,221:351-358. |
| APA | Li, Lian-Zhen,Tu, Chen,Peijnenburg, Willie J. G. M.,Luo, Yong-Ming,&Luo, YM .(2017).Characteristics of cadmium uptake and membrane transport in roots of intact wheat (Triticum aestivum L.) seedlings.ENVIRONMENTAL POLLUTION,221,351-358. |
| MLA | Li, Lian-Zhen,et al."Characteristics of cadmium uptake and membrane transport in roots of intact wheat (Triticum aestivum L.) seedlings".ENVIRONMENTAL POLLUTION 221(2017):351-358. |
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