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题名:
Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods
作者: Wang, Beibei1,2; Liu, Wuxing1; Liu, Xiaoyan1,2; Franks, Ashley E.3; Teng, Ying1; Luo, Yongming1,4
刊名: SCIENCE OF THE TOTAL ENVIRONMENT
ISSN号: 0048-9697
出版日期: 2017-07-15
卷号: 590, 页码:297-303
关键词: DDT-degrading microorganisms ; Microbial community ; Culture-dependent ; Culture-independent ; Community diversity
学科分类: Environmental Sciences & Ecology
DOI: 10.1016/j.scitotenv.2017.03.004
产权排序: [Wang, Beibei; Liu, Wuxing; Liu, Xiaoyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Wang, Beibei; Liu, Xiaoyan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Franks, Ashley E.] La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia; [Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China
通讯作者: Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. liuwuxin@issas.ac.cn
作者部门: 中科院海岸带环境过程与生态修复重点实验室
英文摘要: Microcosms for enrichment of DDT degrading microorganisms were monitored using culture-dependent and -independent methods. Culture dependent methods isolated several strains with DDT degradation potential, Pseudomonas species being the most frequent. One isolate, Streptomyces sp. strain D3, had a degradation rate of 77% with 20 mg L-1 of DDT after 7 days incubation, D3 also had degradation rates of 75% and 30% for PCB77 (3,3',4,4'-tetrachloro biphenyl) and PCNB (pentachloronitrobenzene) respectively. Culture-independent high throughput sequencing identified a different subset of the microbial community within the enrichment microcosms to the culture dependent method. Pseudomonas, the most frequently isolated strain, only represented the 12th most abundant operational taxonomic unit in the sequencing dataset (relative abundance 0.9%). The most frequently observed bacterial genus in the culture-independent analysis did not correspond with those recovered by culture-dependent methods. These results suggested that deep sequencing followed by a targeted isolation approach might provide an advantageous route to bioremediation studies. (C) 2017 Elsevier B.V. All rights reserved.
研究领域[WOS]: Environmental Sciences & Ecology
项目资助者: Key Research Program of the Chinese Academy of Sciences(KFZD-SW-303) ; National Natural Science Foundation of China(41671325 ; U1662110)
关键词[WOS]: ORGANOCHLORINE PESTICIDES ; SEA BACTERIOPLANKTON ; AGRICULTURAL SOILS ; CONTAMINATED SOIL ; DEGRADATION ; CHINA ; WATER ; BIODEGRADATION ; DIVERSITY ; PENTACHLORONITROBENZENE
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000399511800031
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内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/22047
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia
4.Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China

Recommended Citation:
Wang, Beibei,Liu, Wuxing,Liu, Xiaoyan,et al. Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2017,590:297-303.
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