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Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods
Wang, Beibei1,2; Liu, Wuxing1; Liu, Xiaoyan1,2; Franks, Ashley E.3; Teng, Ying1; Luo, Yongming1,4; Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. liuwuxin@issas.ac.cn
2017-07-15
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume590Pages:297-303
Contribution Rank[Wang, Beibei; Liu, Wuxing; Liu, Xiaoyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Wang, Beibei; Liu, Xiaoyan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Franks, Ashley E.] La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia; [Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China
AbstractMicrocosms for enrichment of DDT degrading microorganisms were monitored using culture-dependent and -independent methods. Culture dependent methods isolated several strains with DDT degradation potential, Pseudomonas species being the most frequent. One isolate, Streptomyces sp. strain D3, had a degradation rate of 77% with 20 mg L-1 of DDT after 7 days incubation, D3 also had degradation rates of 75% and 30% for PCB77 (3,3',4,4'-tetrachloro biphenyl) and PCNB (pentachloronitrobenzene) respectively. Culture-independent high throughput sequencing identified a different subset of the microbial community within the enrichment microcosms to the culture dependent method. Pseudomonas, the most frequently isolated strain, only represented the 12th most abundant operational taxonomic unit in the sequencing dataset (relative abundance 0.9%). The most frequently observed bacterial genus in the culture-independent analysis did not correspond with those recovered by culture-dependent methods. These results suggested that deep sequencing followed by a targeted isolation approach might provide an advantageous route to bioremediation studies. (C) 2017 Elsevier B.V. All rights reserved.
KeywordDdt-degrading Microorganisms Microbial Community Culture-dependent Culture-independent Community Diversity
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaEnvironmental Sciences & Ecology
DOI10.1016/j.scitotenv.2017.03.004
Funding OrganizationKey Research Program of the Chinese Academy of Sciences(KFZD-SW-303) ; National Natural Science Foundation of China(41671325 ; U1662110)
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Indexed BySCI
WOS KeywordORGANOCHLORINE PESTICIDES ; SEA BACTERIOPLANKTON ; AGRICULTURAL SOILS ; CONTAMINATED SOIL ; DEGRADATION ; CHINA ; WATER ; BIODEGRADATION ; DIVERSITY ; PENTACHLORONITROBENZENE
SubtypeArticle
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS IDWOS:000399511800031
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/22047
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorLiu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. liuwuxin@issas.ac.cn
Affiliation1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia
4.Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China
Recommended Citation
GB/T 7714
Wang, Beibei,Liu, Wuxing,Liu, Xiaoyan,et al. Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2017,590:297-303.
APA Wang, Beibei.,Liu, Wuxing.,Liu, Xiaoyan.,Franks, Ashley E..,Teng, Ying.,...&Liu, WX .(2017).Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods.SCIENCE OF THE TOTAL ENVIRONMENT,590,297-303.
MLA Wang, Beibei,et al."Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods".SCIENCE OF THE TOTAL ENVIRONMENT 590(2017):297-303.
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