Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods
Wang, Beibei1,2; Liu, Wuxing1; Liu, Xiaoyan1,2; Franks, Ashley E.3; Teng, Ying1; Luo, Yongming1,4; Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. liuwuxin@issas.ac.cn
2017-07-15
发表期刊SCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
卷号590页码:297-303
产权排序[Wang, Beibei; Liu, Wuxing; Liu, Xiaoyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Wang, Beibei; Liu, Xiaoyan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Franks, Ashley E.] La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia; [Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China
摘要Microcosms for enrichment of DDT degrading microorganisms were monitored using culture-dependent and -independent methods. Culture dependent methods isolated several strains with DDT degradation potential, Pseudomonas species being the most frequent. One isolate, Streptomyces sp. strain D3, had a degradation rate of 77% with 20 mg L-1 of DDT after 7 days incubation, D3 also had degradation rates of 75% and 30% for PCB77 (3,3',4,4'-tetrachloro biphenyl) and PCNB (pentachloronitrobenzene) respectively. Culture-independent high throughput sequencing identified a different subset of the microbial community within the enrichment microcosms to the culture dependent method. Pseudomonas, the most frequently isolated strain, only represented the 12th most abundant operational taxonomic unit in the sequencing dataset (relative abundance 0.9%). The most frequently observed bacterial genus in the culture-independent analysis did not correspond with those recovered by culture-dependent methods. These results suggested that deep sequencing followed by a targeted isolation approach might provide an advantageous route to bioremediation studies. (C) 2017 Elsevier B.V. All rights reserved.
关键词Ddt-degrading Microorganisms Microbial Community Culture-dependent Culture-independent Community Diversity
作者部门中科院海岸带环境过程与生态修复重点实验室
学科领域Environmental Sciences & Ecology
DOI10.1016/j.scitotenv.2017.03.004
项目资助者Key Research Program of the Chinese Academy of Sciences(KFZD-SW-303) ; National Natural Science Foundation of China(41671325 ; U1662110)
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收录类别SCI
关键词[WOS]ORGANOCHLORINE PESTICIDES ; SEA BACTERIOPLANKTON ; AGRICULTURAL SOILS ; CONTAMINATED SOIL ; DEGRADATION ; CHINA ; WATER ; BIODEGRADATION ; DIVERSITY ; PENTACHLORONITROBENZENE
文章类型Article
语种英语
WOS研究方向Environmental Sciences & Ecology
WOS记录号WOS:000399511800031
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22047
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. liuwuxin@issas.ac.cn
作者单位1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia
4.Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China
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Wang, Beibei,Liu, Wuxing,Liu, Xiaoyan,et al. Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2017,590:297-303.
APA Wang, Beibei.,Liu, Wuxing.,Liu, Xiaoyan.,Franks, Ashley E..,Teng, Ying.,...&Liu, WX .(2017).Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods.SCIENCE OF THE TOTAL ENVIRONMENT,590,297-303.
MLA Wang, Beibei,et al."Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods".SCIENCE OF THE TOTAL ENVIRONMENT 590(2017):297-303.
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