Purification of quercetin-3-0-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography
Li, Hongjuan1,3,4; Liu, Ying2; Yi, Yuetao1; Miao, Qin1,4; Liu, Sujing1; Zhao, Feng3; Cong, Wei3; Wang, Chunhua3; Xia, Chuanhai2; Xia, CH (reprint author), Ludong Univ, Sch Resources & Environm Engn, Middle Hongqi Rd 186, Yantai 264025, Peoples R China.
2017-03-24
发表期刊JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
卷号1048页码:56-63
产权排序[Li, Hongjuan; Yi, Yuetao; Miao, Qin; Liu, Sujing] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Ying; Xia, Chuanhai] Ludong Univ, Sch Resources & Environm Engn, Middle Hongqi Rd 186, Yantai 264025, Peoples R China; [Li, Hongjuan; Zhao, Feng; Cong, Wei; Wang, Chunhua] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Li, Hongjuan; Miao, Qin] Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
摘要In China, Poacynum hendersonii is frequently used as a substitute for Apoacynum venetum L (Luobuma), which is a famous traditional Chinese medicine. Quercetin-3-0-sophoroside and isoquercitrin are two major flavonoids in Poacynum hendersonii leaves. In this work, a suitable method was established for the large-scale preparation of quercetin-3-0-sophoroside (QOS) and isoquercitrin (ISO) from Poacynum hendersonii leaves using macroporous resin combined with Sephadex LH-20 column chromatography. The adsorption/desorption capacities and desorption ratios of six macroporous resins were evaluated using static experiments. The HPD-300 resin had the best adsorption performance because it had the largest surface area, and was selected for further study. Compared with pseudo -first-order and intraparticle diffusion kinetics models, the pseudo -second-order model could better fit the adsorption kinetics of both QOS and ISO on the HPD-300 resin. In addition, the adsorption isotherms of the two compounds on the HPD-300 resin were fitted well to the Langmuir model. Under optimal conditions, the purities of QOS and ISO in the product were increased from 2.16% and 1.26% to 21.34% and 10.70% with recovery yields of 82.1% and 77.3%, respectively. Subsequently, Sephadex LH-20 column chromatography was employed for improving the purities of the two compounds. After separation by Sephadex LH-20 column chromatography, the purities of QOS and ISO achieved 93.5% and 95.6%, respectively. (c)2017 Elsevier B.V. All rights reserved.
关键词Macroporous Resins Quercetin-3-0-sophoroside Isoquercitrin Purification Poacynum Hendersonii
作者部门海岸带生物学与生物资源利用所重点实验室 
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1016/j.jchromb.2017.01.041
项目资助者Project of the Cultivation Plan of Superior Discipline Talent Teams of Universities in Shandong Province ; Foundation of Shandong Province(2014CGZH1316 ; Shandong Provincial Medical and Health Technology Development Program(2015WS0496) ; ZR2014HP006)
收录类别SCI
关键词[WOS]APOCYNUM-VENETUM L. ; PREPARATIVE SEPARATION ; QUANTITATIVE-ANALYSIS ; ADSORPTION ; FLAVONOIDS ; ANTIOXIDANT ; INJURY ; ACID
文章类型Article
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS记录号WOS:000397686500007
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22013
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Xia, CH (reprint author), Ludong Univ, Sch Resources & Environm Engn, Middle Hongqi Rd 186, Yantai 264025, Peoples R China.
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Ludong Univ, Sch Resources & Environm Engn, Middle Hongqi Rd 186, Yantai 264025, Peoples R China
3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Li, Hongjuan,Liu, Ying,Yi, Yuetao,et al. Purification of quercetin-3-0-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1048:56-63.
APA Li, Hongjuan.,Liu, Ying.,Yi, Yuetao.,Miao, Qin.,Liu, Sujing.,...&Xia, CH .(2017).Purification of quercetin-3-0-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1048,56-63.
MLA Li, Hongjuan,et al."Purification of quercetin-3-0-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1048(2017):56-63.
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