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题名:
Purification of quercetin-3-0-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography
作者: Li, Hongjuan1,3,4; Liu, Ying2; Yi, Yuetao1; Miao, Qin1,4; Liu, Sujing1; Zhao, Feng3; Cong, Wei3; Wang, Chunhua3; Xia, Chuanhai2
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN号: 1570-0232
出版日期: 2017-03-24
卷号: 1048, 页码:56-63
关键词: Macroporous resins ; Quercetin-3-0-sophoroside ; Isoquercitrin ; Purification ; Poacynum hendersonii
学科分类: Biochemistry & Molecular Biology ; Chemistry
DOI: 10.1016/j.jchromb.2017.01.041
产权排序: [Li, Hongjuan; Yi, Yuetao; Miao, Qin; Liu, Sujing] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Ying; Xia, Chuanhai] Ludong Univ, Sch Resources & Environm Engn, Middle Hongqi Rd 186, Yantai 264025, Peoples R China; [Li, Hongjuan; Zhao, Feng; Cong, Wei; Wang, Chunhua] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Li, Hongjuan; Miao, Qin] Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
通讯作者: Xia, CH (reprint author), Ludong Univ, Sch Resources & Environm Engn, Middle Hongqi Rd 186, Yantai 264025, Peoples R China.
作者部门: 海岸带生物学与生物资源利用所重点实验室 
英文摘要: In China, Poacynum hendersonii is frequently used as a substitute for Apoacynum venetum L (Luobuma), which is a famous traditional Chinese medicine. Quercetin-3-0-sophoroside and isoquercitrin are two major flavonoids in Poacynum hendersonii leaves. In this work, a suitable method was established for the large-scale preparation of quercetin-3-0-sophoroside (QOS) and isoquercitrin (ISO) from Poacynum hendersonii leaves using macroporous resin combined with Sephadex LH-20 column chromatography. The adsorption/desorption capacities and desorption ratios of six macroporous resins were evaluated using static experiments. The HPD-300 resin had the best adsorption performance because it had the largest surface area, and was selected for further study. Compared with pseudo -first-order and intraparticle diffusion kinetics models, the pseudo -second-order model could better fit the adsorption kinetics of both QOS and ISO on the HPD-300 resin. In addition, the adsorption isotherms of the two compounds on the HPD-300 resin were fitted well to the Langmuir model. Under optimal conditions, the purities of QOS and ISO in the product were increased from 2.16% and 1.26% to 21.34% and 10.70% with recovery yields of 82.1% and 77.3%, respectively. Subsequently, Sephadex LH-20 column chromatography was employed for improving the purities of the two compounds. After separation by Sephadex LH-20 column chromatography, the purities of QOS and ISO achieved 93.5% and 95.6%, respectively. (c)2017 Elsevier B.V. All rights reserved.
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
项目资助者: Project of the Cultivation Plan of Superior Discipline Talent Teams of Universities in Shandong Province ; Foundation of Shandong Province(2014CGZH1316 ; Shandong Provincial Medical and Health Technology Development Program(2015WS0496) ; ZR2014HP006)
关键词[WOS]: APOCYNUM-VENETUM L. ; PREPARATIVE SEPARATION ; QUANTITATIVE-ANALYSIS ; ADSORPTION ; FLAVONOIDS ; ANTIOXIDANT ; INJURY ; ACID
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000397686500007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/22013
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Ludong Univ, Sch Resources & Environm Engn, Middle Hongqi Rd 186, Yantai 264025, Peoples R China
3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China

Recommended Citation:
Li, Hongjuan,Liu, Ying,Yi, Yuetao,et al. Purification of quercetin-3-0-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1048:56-63.
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文件名: Purification of quercetin-3-0-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography.pdf
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