A group of Sesbania cannabina rhizobia belonging to four recA genotypes of a novel group was further characterized in comparison with the related Ensifer species. They showed 98.2 to 99.9% similarities among themselves and 92.9 to 93.3% similarities with the most related strain Ensifer sojae CCBAU 05684(T) in multilocus sequence analysis of recA, atpD and gin/I. The genome average nucleotide identity values between representative strain YIC4027(T) and the type strains of its closely related species were 81.6 to 88.9%. Identical symbiotic gene (nodA, nodC and nifH) sequences highly similar with those in other Sesbania-nodulating strains (Rhizobium sp. SIN-1, Neorhizobium huautlense SO2(T), Ensifer saheli ORS609(T) and Rhizobium sp. IRBG74) were detected. The representative strain YIC4027(T) could form effective nodules on its original host Sesbania cannabina, but not on Sophora flavescens, Trifolium repens, Glycine max, Glycyrrhiza uralensis, Phaseolus vulgaris or Medicago sativa. The use of lactulose as sole carbon source, possession of C-13:0 2-OH, C-13:1 at 12-13, C-15:1 iso omega 9c, C-17:0 anteiso and C-18:0 iso and absence of C-14:0 anteiso, C-15:0 anteiso and C-18:0 3-OH in fatty acids distinguished the strain YIC4027(T) from the type strains of its closely related species. Based on all the analyses mentioned above, we propose a novel species Ensifer alkalisoli sp. nov. and designate YIC4027(T) (=HAMBI 3655(T)=LMG 29286(T)) as the type strain. The genome size of YIC4027(T) is 5.97 Mbp, comprising 5588 predicted genes, and the DNA G+C content is 62.2 mol%.
WOS Subject Extended:
Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020403)
; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14)
; National Natural Science Foundation of China(31370108
; One Hundred-Talent Plan of Chinese Academy of Sciences, Yantai Science and Technology Project(2013JH021)
; Shandong Independent Innovation and Achievement Transformation Program(2014ZZCX07303)
; (SIP 20150597)
1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Harbin, Peoples R China 3.Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City 11340, DF, Mexico 4.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing, Peoples R China 5.China Agr Univ, Rhizobium Res Ctr, Beijing, Peoples R China
Li, Yan,Yan, Jun,Yu, Bing,et al. Ensifer alkalisoli sp nov isolated from root nodules of Sesbania cannabina grown in saline-alkaline soils[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2016,66:5294-5300.