Transient expression of the enhanced green fluorescence protein (egfp) gene in Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta) with three exogenous promoters
Cui, Yulin; Qu, Lin; Zhao, Jialin; Qin, Song; Qin, S (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China. sqin@yic.ac.cn
发表期刊PHYCOLOGIA
ISSN0031-8884
2016
卷号55期号:5页码:564-567
关键词Enhanced Green Fluorescence Protein Gene Promoter Tetraselmis Subcordiformis Transformation Rate
DOI10.2216/15-136.1
产权排序[Cui, Yulin; Zhao, Jialin; Qin, Song] Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Qu, Lin] State Ocean Adm, Yantai Ocean Environm Monitoring Cent, Yantai 264006, Shandong, Peoples R China; [Zhao, Jialin] Univ Chinese Acad Sci, Beijing 101408, Peoples R China
作者部门海岸带生物学与生物资源利用所重点实验室 
英文摘要A stable transformation system for Tetraselmis subcordiformis had been established, but a low transformation rate limits its application to this alga. As a promoter is a key factor for transgenic research, a suitable promoter could increase the transformation rate. To improve a transformation system for T. subcordiformis, three commonly used promoters for algal transformation were selected. The CMV, CaMV35S and SV40 promoters were transferred to T. subcordiformis by glass-bead agitation and particle bombardment, with the enhanced green fluorescence protein (egfp) gene as the reporter gene. The transformation rate was calculated according to the GFP fluorescence in transformed algae. Results showed that the CaMV 35S promoter transformation efficiency was higher than that of CMV and SV40 promoters. This report will contribute to efforts to establish a high-efficiency transformation system and expands the fundamental research and applications of this alga.
文章类型Article
资助机构National Natural Science Foundation of China(41406192 ; National Key Technology R&D Program of China(2013BAB01B01) ; National High-tech R&D Program of China(2014AA093505) ; Ocean Public Welfare Scientific Research Project, State Oceanic Administration of China(201205027) ; 41176144 ; 41376139)
收录类别SCI
语种英语
关键词[WOS]ALGA PLATYMONAS-SUBCORDIFORMIS ; CHLAMYDOMONAS-REINHARDTII ; NUCLEAR TRANSFORMATION ; PRASINOPHYCEAE ; AGITATION
研究领域[WOS]Plant Sciences ; Marine & Freshwater Biology
WOS记录号WOS:000390261700007
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/19251
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Qin, S (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China. sqin@yic.ac.cn
作者单位1.Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
2.State Ocean Adm, Yantai Ocean Environm Monitoring Cent, Yantai 264006, Shandong, Peoples R China
3.State Ocean Adm, Yantai Ocean Environm Monitoring Cent, Yantai 264006, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
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Cui, Yulin,Qu, Lin,Zhao, Jialin,et al. Transient expression of the enhanced green fluorescence protein (egfp) gene in Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta) with three exogenous promoters[J]. PHYCOLOGIA,2016,55(5):564-567.
APA Cui, Yulin,Qu, Lin,Zhao, Jialin,Qin, Song,&Qin, S .(2016).Transient expression of the enhanced green fluorescence protein (egfp) gene in Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta) with three exogenous promoters.PHYCOLOGIA,55(5),564-567.
MLA Cui, Yulin,et al."Transient expression of the enhanced green fluorescence protein (egfp) gene in Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta) with three exogenous promoters".PHYCOLOGIA 55.5(2016):564-567.
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