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题名:
A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry
作者: Zhang, JJ; Liu, DY; Cao, P; Wang, YJ; Keesing, JK; Jinhua LI(李金花); Chen, LX
刊名: LIMNOLOGY AND OCEANOGRAPHY-METHODS
ISSN号: 1541-5856
出版日期: 2016-10-01
卷号: 14, 期号:10, 页码:623-636
DOI: 10.1002/lom3.10117
产权排序: [Zhang, Jingjing; Liu, Dongyan; Wang, Yujue; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China; [Zhang, Jingjing; Liu, Dongyan; Wang, Yujue; Li, Jinhua; Chen, Lingxin] Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Zhang, Jingjing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cao, Peng] Yantai Entry Exit Inspect & Quarantine Bur, Yantai 264000, Shandong, Peoples R China; [Keesing, John K.] CSIRO Oceans & Atmosphere, Private Bag 5, Wembley, WA 6913, Australia
通讯作者: Liu, DY (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China. ; Liu, DY (reprint author), Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China. Email:dyliu@yic.ac.cn
作者部门: 中科院海岸带环境过程与生态修复重点实验室
英文摘要: A sensitive and specific ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for the purpose of decreasing the detection limit of quantification (LOQ) and reducing sample analysis time. In total, 14 marker pigments were determined and validated using an internal standard (IS) method with trans-8'-Apo-beta-carotenal as the IS. Electrospray ionization in positive mode was applied for the detection of pigments. The 14 types of pigments were separated in less than 15 min and presented good linearity, with the coefficients ranging from 0.9915 to 0.9991 using a UHPLC C8 column. The LOQ attained for 14 pigments were from 0.005 ng mL(-1) to 0.500 ng mL(-1), which enabled quantification of the pigments in seawater at ppt level. Both intra-day and inter-day precisions were less than 15%. The method was validated using seawater samples and the recovery of the analytes ranged from 62.8% to 120.2% at two spiked concentrations. Compared with previous methods, UHPLC-MS/MS method improved the efficiency of qualitative analysis and significantly decreased the detection LOQ for analyzing marker phytoplankton pigments at trace/ultra-trace levels in complex matrices.
研究领域[WOS]: Marine & Freshwater Biology ; Oceanography
项目资助者: Natural Science Foundation of China(41376121) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020405) ; Natural Science Foundation of Shandong Province(JQ201414)
关键词[WOS]: CONTAINING MOBILE PHASES ; MARINE-PHYTOPLANKTON ; EMILIANIA-HUXLEYI ; ANTARCTIC LAKE ; HPLC METHOD ; CHLOROPHYLLS ; CAROTENOIDS ; SEPARATION ; RESOLUTION ; DISTRIBUTIONS
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000386966700001
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内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/17471
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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Recommended Citation:
Zhang, JJ,Liu, DY,Cao, P,et al. A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry[J]. LIMNOLOGY AND OCEANOGRAPHY-METHODS,2016,14(10):623-636.
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