A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry
Zhang, JJ; Liu, DY; Cao, P; Wang, YJ; Keesing, JK; Li, JH; Chen, LX; Liu, DY (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China.; Liu, DY (reprint author), Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China. Email:dyliu@yic.ac.cn
2016-10-01
发表期刊LIMNOLOGY AND OCEANOGRAPHY-METHODS
ISSN1541-5856
卷号14期号:10页码:623-636
产权排序[Zhang, Jingjing; Liu, Dongyan; Wang, Yujue; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China; [Zhang, Jingjing; Liu, Dongyan; Wang, Yujue; Li, Jinhua; Chen, Lingxin] Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Zhang, Jingjing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cao, Peng] Yantai Entry Exit Inspect & Quarantine Bur, Yantai 264000, Shandong, Peoples R China; [Keesing, John K.] CSIRO Oceans & Atmosphere, Private Bag 5, Wembley, WA 6913, Australia
摘要A sensitive and specific ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for the purpose of decreasing the detection limit of quantification (LOQ) and reducing sample analysis time. In total, 14 marker pigments were determined and validated using an internal standard (IS) method with trans-8'-Apo-beta-carotenal as the IS. Electrospray ionization in positive mode was applied for the detection of pigments. The 14 types of pigments were separated in less than 15 min and presented good linearity, with the coefficients ranging from 0.9915 to 0.9991 using a UHPLC C8 column. The LOQ attained for 14 pigments were from 0.005 ng mL(-1) to 0.500 ng mL(-1), which enabled quantification of the pigments in seawater at ppt level. Both intra-day and inter-day precisions were less than 15%. The method was validated using seawater samples and the recovery of the analytes ranged from 62.8% to 120.2% at two spiked concentrations. Compared with previous methods, UHPLC-MS/MS method improved the efficiency of qualitative analysis and significantly decreased the detection LOQ for analyzing marker phytoplankton pigments at trace/ultra-trace levels in complex matrices.
作者部门中科院海岸带环境过程与生态修复重点实验室
DOI10.1002/lom3.10117
项目资助者Natural Science Foundation of China(41376121) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020405) ; Natural Science Foundation of Shandong Province(JQ201414)
收录类别SCI
关键词[WOS]CONTAINING MOBILE PHASES ; MARINE-PHYTOPLANKTON ; EMILIANIA-HUXLEYI ; ANTARCTIC LAKE ; HPLC METHOD ; CHLOROPHYLLS ; CAROTENOIDS ; SEPARATION ; RESOLUTION ; DISTRIBUTIONS
文章类型Article
语种英语
WOS研究方向Marine & Freshwater Biology ; Oceanography
WOS记录号WOS:000386966700001
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/17471
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Liu, DY (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China.; Liu, DY (reprint author), Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China. Email:dyliu@yic.ac.cn
推荐引用方式
GB/T 7714
Zhang, JJ,Liu, DY,Cao, P,et al. A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry[J]. LIMNOLOGY AND OCEANOGRAPHY-METHODS,2016,14(10):623-636.
APA Zhang, JJ.,Liu, DY.,Cao, P.,Wang, YJ.,Keesing, JK.,...&Liu, DY .(2016).A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry.LIMNOLOGY AND OCEANOGRAPHY-METHODS,14(10),623-636.
MLA Zhang, JJ,et al."A highly sensitive method for analyzing marker phytoplankton pigments: Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry".LIMNOLOGY AND OCEANOGRAPHY-METHODS 14.10(2016):623-636.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
A highly sensitive m(440KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, JJ]的文章
[Liu, DY]的文章
[Cao, P]的文章
百度学术
百度学术中相似的文章
[Zhang, JJ]的文章
[Liu, DY]的文章
[Cao, P]的文章
必应学术
必应学术中相似的文章
[Zhang, JJ]的文章
[Liu, DY]的文章
[Cao, P]的文章
相关权益政策
暂无数据
收藏/分享
文件名: A highly sensitive method for analyzing marker phytoplankton pigments Ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。