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题名:
Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa
作者: Cong, M; Zhao, JM; Lu, JS; Ren, ZM; Wu, HF
刊名: CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN号: 0254-4059
出版日期: 2016-09-01
卷号: 34, 期号:5, 页码:1034-1043
关键词: Suaeda salsa ; halophyte ; phytochelatin synthase (PCS) ; homologous cloning ; heavy metal ; tissue distribution
DOI: 10.1007/s00343-016-4382-0
产权排序: [Cong Ming; Zhao Jianmin; Wu Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Lu Jiasen; Ren Zhiming] Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
通讯作者: Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
作者部门: 中科院海岸带环境过程与生态修复重点实验室
英文摘要: The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity. Since phytochelatins can effectively chelate heavy metals, it was hypothesized that S. salsa possessed a phytochelatin synthase (PCS) gene. In the present study, the cDNA of PCS was obtained from S. salsa (designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends (RACE). A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides, encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain. A similarity analysis suggested that SsPCS shared up to a 58.6% identity with other PCS proteins and clustered with PCS proteins from eudicots. There was a new kind of metal ion sensor motif in its C-terminal domain. The SsPCS transcript was more highly expressed in elongated and fibered roots and stems (P < 0.05) than in leaves. Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS (P < 0.05). To the best of our knowledge, SsPCS is the second PCS gene cloned from a halophyte, and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila. This study provided a new view of halophyte PCS genes in heavy metal tolerance.
研究领域[WOS]: Marine & Freshwater Biology ; Oceanography
项目资助者: 100 Talents Program of the Chinese Academy of Sciences ; Key Technology R&D Program of Shandong Province(2012GGA06032)
关键词[WOS]: HEAVY-METALS ; DUNALIELLA-TERTIOLECTA ; CADMIUM EXPOSURE ; EISENIA-FOETIDA ; ARABIDOPSIS ; PROTEIN ; ACCUMULATION ; RESPONSES ; DOMAIN ; PLANTS
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000379737000015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/17108
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc
2.Yantai Univ, Sch Biol

Recommended Citation:
Cong, M,Zhao, JM,Lu, JS,et al. Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1034-1043.
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文件名: Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa.pdf
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