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Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa
Cong, M; Zhao, JM; Lu, JS; Ren, ZM; Wu, HF; Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
2016-09-01
Volume34Issue:5Pages:1034-1043
KeywordSuaeda Salsa Halophyte Phytochelatin Synthase (Pcs) Homologous Cloning Heavy Metal Tissue Distribution
DOI10.1007/s00343-016-4382-0
Contribution Rank[Cong Ming; Zhao Jianmin; Wu Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Lu Jiasen; Ren Zhiming] Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
Department中科院海岸带环境过程与生态修复重点实验室
AbstractThe halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity. Since phytochelatins can effectively chelate heavy metals, it was hypothesized that S. salsa possessed a phytochelatin synthase (PCS) gene. In the present study, the cDNA of PCS was obtained from S. salsa (designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends (RACE). A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides, encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain. A similarity analysis suggested that SsPCS shared up to a 58.6% identity with other PCS proteins and clustered with PCS proteins from eudicots. There was a new kind of metal ion sensor motif in its C-terminal domain. The SsPCS transcript was more highly expressed in elongated and fibered roots and stems (P < 0.05) than in leaves. Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS (P < 0.05). To the best of our knowledge, SsPCS is the second PCS gene cloned from a halophyte, and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila. This study provided a new view of halophyte PCS genes in heavy metal tolerance.
Funding Organization100 Talents Program of the Chinese Academy of Sciences ; Key Technology R&D Program of Shandong Province(2012GGA06032)
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordHEAVY-METALS ; DUNALIELLA-TERTIOLECTA ; CADMIUM EXPOSURE ; EISENIA-FOETIDA ; ARABIDOPSIS ; PROTEIN ; ACCUMULATION ; RESPONSES ; DOMAIN ; PLANTS
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS IDWOS:000379737000015
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/17108
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
Affiliation1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc
2.Yantai Univ, Sch Biol
Recommended Citation
GB/T 7714
Cong, M,Zhao, JM,Lu, JS,et al. Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1034-1043.
APA Cong, M,Zhao, JM,Lu, JS,Ren, ZM,Wu, HF,&Wu, HF .(2016).Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1034-1043.
MLA Cong, M,et al."Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1034-1043.
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