Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa | |
Cong, M ; Zhao, JM; Lu, JS; Ren, ZM; Wu, HF; Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
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| Source Publication | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 2016-09-01 | |
| Volume | 34Issue:5Pages:1034-1043 |
| Keyword | Suaeda Salsa Halophyte Phytochelatin Synthase (Pcs) Homologous Cloning Heavy Metal Tissue Distribution |
| DOI | 10.1007/s00343-016-4382-0 |
| Contribution Rank | [Cong Ming; Zhao Jianmin; Wu Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Lu Jiasen; Ren Zhiming] Yantai Univ, Sch Biol, Yantai 264005, Peoples R China |
| Department | 中科院海岸带环境过程与生态修复重点实验室 |
| Abstract | The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity. Since phytochelatins can effectively chelate heavy metals, it was hypothesized that S. salsa possessed a phytochelatin synthase (PCS) gene. In the present study, the cDNA of PCS was obtained from S. salsa (designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends (RACE). A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides, encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain. A similarity analysis suggested that SsPCS shared up to a 58.6% identity with other PCS proteins and clustered with PCS proteins from eudicots. There was a new kind of metal ion sensor motif in its C-terminal domain. The SsPCS transcript was more highly expressed in elongated and fibered roots and stems (P < 0.05) than in leaves. Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS (P < 0.05). To the best of our knowledge, SsPCS is the second PCS gene cloned from a halophyte, and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila. This study provided a new view of halophyte PCS genes in heavy metal tolerance. |
| Funding Organization | 100 Talents Program of the Chinese Academy of Sciences ; Key Technology R&D Program of Shandong Province(2012GGA06032) |
| Subtype | Article |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | HEAVY-METALS ; DUNALIELLA-TERTIOLECTA ; CADMIUM EXPOSURE ; EISENIA-FOETIDA ; ARABIDOPSIS ; PROTEIN ; ACCUMULATION ; RESPONSES ; DOMAIN ; PLANTS |
| WOS Research Area | Marine & Freshwater Biology ; Oceanography |
| WOS ID | WOS:000379737000015 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/17108 |
| Collection | 中科院海岸带环境过程与生态修复重点实验室 |
| Corresponding Author | Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn |
| Affiliation | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc 2.Yantai Univ, Sch Biol |
| Recommended Citation GB/T 7714 | Cong, M,Zhao, JM,Lu, JS,et al. Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1034-1043. |
| APA | Cong, M,Zhao, JM,Lu, JS,Ren, ZM,Wu, HF,&Wu, HF .(2016).Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1034-1043. |
| MLA | Cong, M,et al."Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1034-1043. |
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| Homologous cloning, （435KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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