Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa
Cong, M; Zhao, JM; Lu, JS; Ren, ZM; Wu, HF; Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
2016-09-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
卷号34期号:5页码:1034-1043
产权排序[Cong Ming; Zhao Jianmin; Wu Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Lu Jiasen; Ren Zhiming] Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
摘要The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity. Since phytochelatins can effectively chelate heavy metals, it was hypothesized that S. salsa possessed a phytochelatin synthase (PCS) gene. In the present study, the cDNA of PCS was obtained from S. salsa (designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends (RACE). A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides, encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain. A similarity analysis suggested that SsPCS shared up to a 58.6% identity with other PCS proteins and clustered with PCS proteins from eudicots. There was a new kind of metal ion sensor motif in its C-terminal domain. The SsPCS transcript was more highly expressed in elongated and fibered roots and stems (P < 0.05) than in leaves. Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS (P < 0.05). To the best of our knowledge, SsPCS is the second PCS gene cloned from a halophyte, and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila. This study provided a new view of halophyte PCS genes in heavy metal tolerance.
关键词Suaeda Salsa Halophyte Phytochelatin Synthase (Pcs) Homologous Cloning Heavy Metal Tissue Distribution
作者部门中科院海岸带环境过程与生态修复重点实验室
DOI10.1007/s00343-016-4382-0
项目资助者100 Talents Program of the Chinese Academy of Sciences ; Key Technology R&D Program of Shandong Province(2012GGA06032)
收录类别SCI
关键词[WOS]HEAVY-METALS ; DUNALIELLA-TERTIOLECTA ; CADMIUM EXPOSURE ; EISENIA-FOETIDA ; ARABIDOPSIS ; PROTEIN ; ACCUMULATION ; RESPONSES ; DOMAIN ; PLANTS
文章类型Article
语种英语
WOS研究方向Marine & Freshwater Biology ; Oceanography
WOS记录号WOS:000379737000015
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/17108
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc
2.Yantai Univ, Sch Biol
推荐引用方式
GB/T 7714
Cong, M,Zhao, JM,Lu, JS,et al. Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1034-1043.
APA Cong, M,Zhao, JM,Lu, JS,Ren, ZM,Wu, HF,&Wu, HF .(2016).Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1034-1043.
MLA Cong, M,et al."Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1034-1043.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Homologous cloning, (435KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cong, M]的文章
[Zhao, JM]的文章
[Lu, JS]的文章
百度学术
百度学术中相似的文章
[Cong, M]的文章
[Zhao, JM]的文章
[Lu, JS]的文章
必应学术
必应学术中相似的文章
[Cong, M]的文章
[Zhao, JM]的文章
[Lu, JS]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。