Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa

doi:10.1007/s00343-016-4382-0

	Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa
	Cong, M ; Zhao, JM ; Lu, JS ; Ren, ZM ; Wu, HF ; Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
	2016-09-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	34 期号:5 页码:1034-1043
产权排序	[Cong Ming; Zhao Jianmin; Wu Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Lu Jiasen; Ren Zhiming] Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
摘要	The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity. Since phytochelatins can effectively chelate heavy metals, it was hypothesized that S. salsa possessed a phytochelatin synthase (PCS) gene. In the present study, the cDNA of PCS was obtained from S. salsa (designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends (RACE). A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides, encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain. A similarity analysis suggested that SsPCS shared up to a 58.6% identity with other PCS proteins and clustered with PCS proteins from eudicots. There was a new kind of metal ion sensor motif in its C-terminal domain. The SsPCS transcript was more highly expressed in elongated and fibered roots and stems (P < 0.05) than in leaves. Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS (P < 0.05). To the best of our knowledge, SsPCS is the second PCS gene cloned from a halophyte, and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila. This study provided a new view of halophyte PCS genes in heavy metal tolerance.
关键词	Suaeda Salsa Halophyte Phytochelatin Synthase (Pcs) Homologous Cloning Heavy Metal Tissue Distribution
作者部门	中科院海岸带环境过程与生态修复重点实验室
DOI	10.1007/s00343-016-4382-0
项目资助者	100 Talents Program of the Chinese Academy of Sciences ; Key Technology R&D Program of Shandong Province(2012GGA06032)
收录类别	SCI
关键词[WOS]	HEAVY-METALS ; DUNALIELLA-TERTIOLECTA ; CADMIUM EXPOSURE ; EISENIA-FOETIDA ; ARABIDOPSIS ; PROTEIN ; ACCUMULATION ; RESPONSES ; DOMAIN ; PLANTS
文章类型	Article
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS记录号	WOS:000379737000015
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17108
专题	中科院海岸带环境过程与生态修复重点实验室
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
作者单位	1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc 2.Yantai Univ, Sch Biol
推荐引用方式 GB/T 7714	Cong, M,Zhao, JM,Lu, JS,et al. Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1034-1043.
APA	Cong, M,Zhao, JM,Lu, JS,Ren, ZM,Wu, HF,&Wu, HF .(2016).Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1034-1043.
MLA	Cong, M,et al."Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1034-1043.

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