Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line

doi:10.3892/or.2016.4844

YIC-IR > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源保护实验室

	Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line
	Wang, CX ; Xu, WJ ; Hao, WJ ; Wang, BS ; Zheng, QS ; Zheng, QS (reprint author), Binzhou Med Univ, Yantai 264003, Shandong, Peoples R China. wang617424@163.com ; zqsyt@sohu.com
发表期刊	ONCOLOGY REPORTS
ISSN	1021-335X
	2016-08-01
卷号	36 期号:2 页码:1150-1156
关键词	Alternol Melanoma Cell Proliferation Differentiation Tyrosinase Melanin
DOI	10.3892/or.2016.4844
产权排序	[Wang, Caixia; Xu, Wenjuan; Hao, Wenjin; Wang, Bingsheng; Zheng, Qiusheng] Binzhou Med Univ, Yantai 264003, Shandong, Peoples R China; [Wang, Caixia] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Shandong, Peoples R China; [Wang, Caixia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	High malignant potential and low susceptibility to treatment are characteristics of malignant melanoma. Alternol, a novel compound purified from microbial fermentation products obtained from the bark of the yew tree, exhibits a variety of antitumor activities. Based on these findings, the aim of the present study was to extend the knowledge on the antineoplastic effect of alternol in the mouse melanoma B16F0 cell line. Alternol significantly inhibited the proliferation and colony formation of B16F0 cells in a dose-dependent manner as detected by MTT and soft agar colony formation assays. NaOH alkaline lysis and oxidation of Dopa indicated that alternol enhanced the melanin content and tyrosinase activity of the B16F0 cells and results also showed a dose-response relationship. Morphologic changes accompanied by extended dendrites were discovered in the B16F0 cells after treatment with alternol. Furthermore, the mRNA levels of tyrosinase, Trpl and Trp2 were increased by alternol. Our results confirmed that alternol possesses marked antineoplastic properties against melanoma cells, indicating that this microbial fermentation product is a promising agent for the differentiation therapy of cancer. The inhibition of cell proliferation and colony formation by alternol was associated with both cytotoxicity and induction of differentiation.
文章类型	Article
资助机构	National Natural Science Foundation of China(31471338) ; Binzhou Medical University(BY2014KYQD01) ; Xinjiang Production and Construction Corps Funds for Innovation Team in Key Areas(2015BD005)
收录类别	SCI
语种	英语
关键词[WOS]	SKIN PIGMENTATION ; COMPOUND ALTERNOL ; RETINOIC ACID ; CANCER-CELLS ; APOPTOSIS ; MELANOCYTES ; GROWTH ; ACTIVATION ; EXPRESSION ; LEUKEMIA
研究领域[WOS]	Oncology
WOS记录号	WOS:000379248700067
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17103
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Wang, BS; Zheng, QS (reprint author), Binzhou Med Univ, Yantai 264003, Shandong, Peoples R China. wang617424@163.com; zqsyt@sohu.com
作者单位	1.Binzhou Med Univ 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat 3.Univ Chinese Acad Sci
推荐引用方式 GB/T 7714	Wang, CX,Xu, WJ,Hao, WJ,et al. Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line[J]. ONCOLOGY REPORTS,2016,36(2):1150-1156.
APA	Wang, CX.,Xu, WJ.,Hao, WJ.,Wang, BS.,Zheng, QS.,...&zqsyt@sohu.com.(2016).Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line.ONCOLOGY REPORTS,36(2),1150-1156.
MLA	Wang, CX,et al."Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line".ONCOLOGY REPORTS 36.2(2016):1150-1156.