YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method
Liu, ZY; Dong, ZJ; Liu, DY; Dong, ZJ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China. zjdong@yic.ac.cn
Source PublicationMITOCHONDRIAL DNA
ISSN1940-1736
2016
Volume27Issue:4Pages:2318-2322
KeywordCyanea Nozakii Cytochrome c Oxidase Subunit i Jellyfish Blooms Lamp Mitochondrial Dna
DOI10.3109/19401736.2015.1022762
Contribution Rank[Liu, Zhongyuan; Dong, Zhijun; Liu, Dongyan] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Shandong, Peoples R China; [Liu, Zhongyuan] Univ Chinese Acad Sci, Beijing, Peoples R China
Department中科院海岸带环境过程与生态修复重点实验室
AbstractBlooms of the harmful jellyfish Cyanea nozakii, which are a severe nuisance to fisheries and tourisms, frequently occur in the northern East China Sea, Yellow Sea, and Bohai Sea. To provide early warning of this species, a simple and effective molecular method for identifying C. nozakii was developed using the loop-mediated isothermal amplification method (LAMP). The LAMP assay is highly specific and uses a set of four primers that target six different regions on the mitochondrial cytochrome c oxidase subunit I (COI) gene of C. nozakii. The amplification conditions, including the dNTP and betaine concentrations, the inner primer to outer primer concentration ratio, reaction time and temperature, were optimized. The LAMP assay amplified DNA extracted from tissue samples of C. nozakii but did not amplify DNA from other common scyphozoans and hydrozoans collected in the same region. In addition, the LAMP assay was more sensitive than conventional PCR. Therefore, the established LAMP assay is a sensitive, specific, fast, and easily performed method for detection of C. nozakii at different stages in their life cycle.
Funding OrganizationNational Natural Science Foundation of China(41206086) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA05130703 ; Research Encouragement Foundation of Excellent Midlife-Youth Scientists of Shandong Province(BS2011HZ023) ; XDA11020405)
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordSCYPHOZOAN JELLYFISH ; BLOOMS ; PCR ; SEQUENCES ; PRIMERS ; AURELIA ; POLYPS ; WATERS ; SEA
WOS Research AreaGenetics & Heredity
WOS IDWOS:000377956300004
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/17090
Collection中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
Corresponding AuthorDong, ZJ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China. zjdong@yic.ac.cn
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia
2.Univ Chinese Acad Sci
Recommended Citation
GB/T 7714
Liu, ZY,Dong, ZJ,Liu, DY,et al. Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method[J]. MITOCHONDRIAL DNA,2016,27(4):2318-2322.
APA Liu, ZY,Dong, ZJ,Liu, DY,&Dong, ZJ .(2016).Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method.MITOCHONDRIAL DNA,27(4),2318-2322.
MLA Liu, ZY,et al."Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method".MITOCHONDRIAL DNA 27.4(2016):2318-2322.
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