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| Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method | |
Liu, ZY ; Dong, ZJ; Liu, DY; Dong, ZJ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China. zjdong@yic.ac.cn
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| 发表期刊 | MITOCHONDRIAL DNA
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| ISSN | 1940-1736 |
| 2016 | |
| 卷号 | 27期号:4页码:2318-2322 |
| 关键词 | Cyanea Nozakii Cytochrome c Oxidase Subunit i Jellyfish Blooms Lamp Mitochondrial Dna |
| DOI | 10.3109/19401736.2015.1022762 |
| 产权排序 | [Liu, Zhongyuan; Dong, Zhijun; Liu, Dongyan] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Shandong, Peoples R China; [Liu, Zhongyuan] Univ Chinese Acad Sci, Beijing, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Blooms of the harmful jellyfish Cyanea nozakii, which are a severe nuisance to fisheries and tourisms, frequently occur in the northern East China Sea, Yellow Sea, and Bohai Sea. To provide early warning of this species, a simple and effective molecular method for identifying C. nozakii was developed using the loop-mediated isothermal amplification method (LAMP). The LAMP assay is highly specific and uses a set of four primers that target six different regions on the mitochondrial cytochrome c oxidase subunit I (COI) gene of C. nozakii. The amplification conditions, including the dNTP and betaine concentrations, the inner primer to outer primer concentration ratio, reaction time and temperature, were optimized. The LAMP assay amplified DNA extracted from tissue samples of C. nozakii but did not amplify DNA from other common scyphozoans and hydrozoans collected in the same region. In addition, the LAMP assay was more sensitive than conventional PCR. Therefore, the established LAMP assay is a sensitive, specific, fast, and easily performed method for detection of C. nozakii at different stages in their life cycle. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China(41206086) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA05130703 ; Research Encouragement Foundation of Excellent Midlife-Youth Scientists of Shandong Province(BS2011HZ023) ; XDA11020405) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SCYPHOZOAN JELLYFISH ; BLOOMS ; PCR ; SEQUENCES ; PRIMERS ; AURELIA ; POLYPS ; WATERS ; SEA |
| 研究领域[WOS] | Genetics & Heredity |
| WOS记录号 | WOS:000377956300004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/17090 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
| 通讯作者 | Dong, ZJ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China. zjdong@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia 2.Univ Chinese Acad Sci |
| 推荐引用方式 GB/T 7714 | Liu, ZY,Dong, ZJ,Liu, DY,et al. Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method[J]. MITOCHONDRIAL DNA,2016,27(4):2318-2322. |
| APA | Liu, ZY,Dong, ZJ,Liu, DY,&Dong, ZJ .(2016).Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method.MITOCHONDRIAL DNA,27(4),2318-2322. |
| MLA | Liu, ZY,et al."Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method".MITOCHONDRIAL DNA 27.4(2016):2318-2322. |
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