| Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators | |
Cui, YL ; Zhao, JL; Hou, SC; Qin, S; Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Chunhui Rd 17, Yantai 264003, Shandong, Peoples R China. yulincui@yic.ac.cn; sqin@yic.ac.cn
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| 发表期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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| ISSN | 0959-3993 |
| 2016-05-01 | |
| 卷号 | 32期号:5 |
| 关键词 | Tetraselmis Subcordiformis Chloroplast Promoters Bombardment |
| DOI | 10.1007/s11274-016-2039-y |
| 产权排序 | [Cui, Yulin; Zhao, Jialin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Chunhui Rd 17, Yantai 264003, Shandong, Peoples R China; [Zhao, Jialin] Univ Chinese Acad Sci, Yuquan Rd 19, Beijing 100049, Peoples R China; [Hou, Shichang] Yantai Univ, Coll Life Sci, Qingquan Rd 30, Yantai 264005, Shandong, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | On the basis of fundamental genetic transformation technologies, the goal of this study was to optimize Tetraselmis subcordiformis chloroplast transformation through the use of endogenous regulators. The genes rrn16S, rbcL, psbA, and psbC are commonly highly expressed in chloroplasts, and the regulators of these genes are often used in chloroplast transformation. For lack of a known chloroplast genome sequence, the genome-walking method was used here to obtain full sequences of T. subcordiformis endogenous regulators. The resulting regulators, including three promoters, two terminators, and a ribosome combination sequence, were inserted into the previously constructed plasmid pPSC-R, with the egfp gene included as a reporter gene, and five chloroplast expression vectors prepared. These vectors were successfully transformed into T. subcordiformis by particle bombardment and the efficiency of each vector tested by assessing EGFP fluorescence via microscopy. The results showed that these vectors exhibited higher efficiency than the former vector pPSC-G carrying exogenous regulators, and the vector pRFA with Prrn, psbA-5'RE, and TpsbA showed the highest efficiency. This research provides a set of effective endogenous regulators for T. subcordiformis and will facilitate future fundamental studies of this alga. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China(41406192 ; Science Foundation of the Chinese Academy of Sciences(XDA1102040300) ; 41176144 ; 41376139) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ALGA PLATYMONAS-SUBCORDIFORMIS ; PLASTID TRANSFORMATION ; VECTOR ; CONSTRUCTION ; SEQUENCES ; ELEMENTS ; TOBACCO ; OPERON |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000375089400012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/17058 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Chunhui Rd 17, Yantai 264003, Shandong, Peoples R China. yulincui@yic.ac.cn; sqin@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat 2.Univ Chinese Acad Sci 3.Yantai Univ, Coll Life Sci |
| 推荐引用方式 GB/T 7714 | Cui, YL,Zhao, JL,Hou, SC,et al. Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2016,32(5). |
| APA | Cui, YL,Zhao, JL,Hou, SC,Qin, S,Qin, S ,&sqin@yic.ac.cn.(2016).Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,32(5). |
| MLA | Cui, YL,et al."Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 32.5(2016). |
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| Enhanced green fluor（645KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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