| Facile Construction of Near Infrared Fluorescence Nanoprobe with Amphiphilic Protein-Polymer Bioconjugate for Targeted Cell Imaging | |
| Liu, Zhongyun1,2,3; Chen, Na4; Dong, Chunhong1,2; Li, Wei5; Guo, Weisheng1,2; Wang, Hanjie1,2; Wang, Sheng1,2; Tan, Jian5; Tu, Yu4; Chang, Jin1,2; Tu, Y (reprint author), Soochow Univ, Jiangsu Prov Key Lab Radiat Med & Protect, Sch Radiat Med & Hlth, Dept Med Radioprotect, Suzhou 200072, Peoples R China. tuyu@suda.edu.cn; jinchang@tju.edu.cn | |
| 发表期刊 | ACS APPLIED MATERIALS & INTERFACES
 |
| ISSN | 1944-8244 |
| 2015-09-02 | |
| 卷号 | 7期号:34页码:18997-19005 |
| 关键词 | Protein-polymer Conjugate Bsa Self-assembly Cuins2/zns Quantum Dots Fluorescence Nanoprobe |
| DOI | 10.1021/acsami.5b05406 |
| 产权排序 | [Liu, Zhongyun; Dong, Chunhong; Guo, Weisheng; Wang, Hanjie; Wang, Sheng; Chang, Jin] Tianjin Univ, Sch Life Sci, Tianjin 300072, Peoples R China; [Liu, Zhongyun; Dong, Chunhong; Guo, Weisheng; Wang, Hanjie; Wang, Sheng; Chang, Jin] Tianjin Engn Ctr Micronano Biomat & Detect Treatm, Tianjin 300072, Peoples R China; [Liu, Zhongyun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Chen, Na; Tu, Yu] Soochow Univ, Jiangsu Prov Key Lab Radiat Med & Protect, Sch Radiat Med & Hlth, Dept Med Radioprotect, Suzhou 200072, Peoples R China; [Li, Wei; Tan, Jian] Tianjin Med Univ, Gen Hosp, Dept Nucl Med, Tianjin 300052, Peoples R China |
| 作者部门 | 山东省海岸带环境工程技术研究中心 |
| 英文摘要 | A simple, straightforward, and reproducible strategy for the construction of a near-infrared (NIR) fluorescence nanoprobe was developed by coating CuInS2/ZnS quantum dots (CIS/ZnS QDs) with a novel amphiphilic bioconjugate. The amphiphilic bioconjugate with a tailor-designed structure of bovine serum albumin (BSA) as the hydrophilic segment and poly(e-caprolactone) (PCL) as the hydrophobic part was fabricated by chemical coupling the hydrophobic polymer chain to BSA via the maleimide sulfhydryl reaction. By incorporating CIS/ZnS QDs into the hydrophobic cores of the self-assembly of BSA-PCL conjugate, the constructed NIR fluorescence nanoprobe exhibited excellent fluorescent properties over a wide pH range (pH 3-10) and a good colloidal stability in PBS buffer (pH = 7.4) with or without 10% fetal bovine serum. The presence of the outer BSA shell effectively reduced the nonspecific cellular binding and imparted high biocompatibility and low-toxicity to the probe. Moreover, the NIR fluorescence nanoprobe could be functionalized by conjugating cyclic Arg-Gly-Asp (cRGD) peptide, and the decorated nanoprobe was shown to be highly selective for targeted integrin alpha(v)beta(3)-overexpressed tumor cell imaging. The feasibility of the constructed NIR fluorescence probe in vivo application was further investigated and the results demonstrated its great potential for in vivo imaging. This developed protocol for phase transfer of the CIS/ZnS QDs was universal and applicable to other nanoparticles stabilized with hydrophobic ligands. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China(51373117 ; Tianjin Natural Science Foundation(13JCZDJC33200) ; Tianjin Research Program of Application Foundation and Advanced Technology(12JCZDJC26000) ; Doctoral Base Foundation of Educational Ministry of China(20120032110027) ; Graduate Student Innovation Project of Jiangsu Province(CXZZ13_0825) ; 51303126) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | BOVINE SERUM-ALBUMIN ; POROUS SILICON NANOPARTICLES ; CUINS2/ZNS QUANTUM DOTS ; IN-VIVO ; DRUG-DELIVERY ; PROBE ; SYSTEMS |
| 研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
| WOS记录号 | WOS:000360868700015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/13818 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
| 通讯作者 | Tu, Y (reprint author), Soochow Univ, Jiangsu Prov Key Lab Radiat Med & Protect, Sch Radiat Med & Hlth, Dept Med Radioprotect, Suzhou 200072, Peoples R China. tuyu@suda.edu.cn; jinchang@tju.edu.cn |
| 作者单位 | 1.Tianjin Univ, Sch Life Sci, Tianjin 300072, Peoples R China 2.Tianjin Engn Ctr Micronano Biomat & Detect Treatm, Tianjin 300072, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 4.Soochow Univ, Jiangsu Prov Key Lab Radiat Med & Protect, Sch Radiat Med & Hlth, Dept Med Radioprotect, Suzhou 200072, Peoples R China 5.Tianjin Med Univ, Gen Hosp, Dept Nucl Med, Tianjin 300052, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhongyun,Chen, Na,Dong, Chunhong,et al. Facile Construction of Near Infrared Fluorescence Nanoprobe with Amphiphilic Protein-Polymer Bioconjugate for Targeted Cell Imaging[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(34):18997-19005. |
| APA | Liu, Zhongyun.,Chen, Na.,Dong, Chunhong.,Li, Wei.,Guo, Weisheng.,...&jinchang@tju.edu.cn.(2015).Facile Construction of Near Infrared Fluorescence Nanoprobe with Amphiphilic Protein-Polymer Bioconjugate for Targeted Cell Imaging.ACS APPLIED MATERIALS & INTERFACES,7(34),18997-19005. |
| MLA | Liu, Zhongyun,et al."Facile Construction of Near Infrared Fluorescence Nanoprobe with Amphiphilic Protein-Polymer Bioconjugate for Targeted Cell Imaging".ACS APPLIED MATERIALS & INTERFACES 7.34(2015):18997-19005. |
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