| Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos | |
Mao, Z. Z.1; Zhang, L. L.1,2; Xu, X. Z.; Liu, Q. H.1,2; Li, J.1 ; Ma, D. Y.1; Xu, S. H.1; Xue, Y. P.; Xue, Q. Z.3
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| 发表期刊 | THERIOGENOLOGY
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| 2008-10-15 | |
| 卷号 | 70期号:7页码:1086-1092 |
| 关键词 | Red Seabream Embryo Cryoprotectant Toxicity Permeable And non-Permeable |
| 产权排序 | Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D;Chinese Acad Sci, Grad Sch;Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev |
| 通讯作者 | Li, J, Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | The objectives were to investigate the effect of cryoprotectants on the hatching rate of red seabream embryos. Heart-beat embryos were immersed in: five permeable cryoprotectants, dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), 1,2-propylene glycol (PG), and ethylene glycol (EG). in concentrations of 5-30% for 10, 30, or 60 min; and two non-permeable cryoprotectants: polyvinylpyrrolidone (PVP), and sucrose (in concentrations of 5-20% for 10 or 30 min). The embryos were then washed and incubated in filtered seawater until hatching occurred. The hatching rate of the embryos treated with permeable cryoprotectants decreased (P < 0.05) with increased concentration and duration of exposure. In addition, PG was the least toxic permeable cryoprotectant, followed by DMSO and EG, whereas Gly and MeOH were the most toxic. At a concentration of 15% and 30 min exposure, the hatching rate of the embryos immersed in PG was 93.3 +/- 7.0% (mean +/- S.D.), however. in DMSO. EG, Gly. and MeOH, it was 82.7 +/- 10.4, 22.0 +/- 5.7, 0.0 +/- 0.0, and 0.0 +/- 0.0%, respectively. Hatching rate of embryos treated with PVP decreased (P < 0.05) with the increase of concentration and exposure time, whereas for embryos treated with sucrose, there was no significant decrease in comparison with the control at the concentrations used. (C) 2008 Elsevier Inc. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [30571427] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PARALICHTHYS-OLIVACEUS EMBRYOS ; ZEBRAFISH DANIO-RERIO ; SPARUS-AURATA EMBRYOS ; CHILLING SENSITIVITY ; SCOPHTHALMUS-MAXIMUS ; BRACHYDANIO-RERIO ; CRASSOSTREA-GIGAS ; CRYOPRESERVATION ; TOXICITY ; VITRIFICATION |
| 研究领域[WOS] | Reproductive Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000259750000009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/1220 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 中国科学院烟台海岸带研究所知识产出 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, Z. Z.,Zhang, L. L.,Xu, X. Z.,et al. Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos[J]. THERIOGENOLOGY,2008,70(7):1086-1092. |
| APA | Mao, Z. Z..,Zhang, L. L..,Xu, X. Z..,Liu, Q. H..,Li, J..,...&Xue, Q. Z..(2008).Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos.THERIOGENOLOGY,70(7),1086-1092. |
| MLA | Mao, Z. Z.,et al."Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos".THERIOGENOLOGY 70.7(2008):1086-1092. |
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